Bactericidal Activity of Serum by Brucella Abortus RB51 Outer Membrane Protein's Combined by Brucella Abortus S99 Lipopolysaccharide Induction.

Q3 Biochemistry, Genetics and Molecular Biology

Avicenna journal of medical biotechnology Pub Date : 2024-07-01 DOI:10.18502/ajmb.v16i3.15745

Behnam Hajizadeh Sisakht, Mansoor Khaledi, Hamed Afkhami, Saber Rouhi, Saeed Sepehrnia, Vahideh Fanaee, Hannaneh Karimi, Yalda Malekzadegan, Javad Fathi, Mahdi S Sadati

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Abstract

Background: Brucellosis vaccines are designed to induce cellular immunity. An effective brucellosis vaccine could induce both cellular and humoral immunity. Serum Bactericidal Assay (SBA) is an important method for determining vaccine humoral immunity. This study is the first to observe humoral immunity in brucellosis by SBA.

Methods: Extracted Brucella abortus (B. abortus) Lipopolysaccharide (LPS) and Outer Membrane Proteins (OMPs) were injected into rabbits. Group 1 was injected with 25 μg of LPS, Group 2 was injected with 50 μg of OMPs, and Group 3 was injected with 1 ml of combined vaccine, 3 times every 2 weeks. The groups were challenged with B. abortus 544 in the second injection. Sera were separated 2 weeks after the last injection. SBA was performed, and each well was streak-cultured into a plate of Brucella agar. A colony count was done for each plate.

Results: Results have shown, the third injection of the combined vaccine had the highest titer of $\frac{1}{64}$ , and the efficacy of the vaccine was 87.71%.

Conclusion: As a conclusion, the results of this study showed that LPS and OMP's from B. abortus can provide acceptable immunity.

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流产布鲁氏菌 RB51 外膜蛋白结合流产布鲁氏菌 S99 脂多糖诱导血清的杀菌活性

背景：布鲁氏菌病疫苗旨在诱导细胞免疫。有效的布鲁氏菌病疫苗可同时诱导细胞免疫和体液免疫。血清杀菌测定（SBA）是确定疫苗体液免疫的重要方法。本研究首次通过 SBA 观察布鲁氏菌病的体液免疫：方法：将提取的流产布鲁氏菌（B. abortus）脂多糖（LPS）和外膜蛋白（OMPs）注射到兔子体内。第 1 组注射 25 μg LPS，第 2 组注射 50 μg OMPs，第 3 组注射 1 ml 混合疫苗，每 2 周注射 3 次。第 2 次注射时，各组均受到流产 B. 544 的挑战。最后一次注射 2 周后分离血清。进行 SBA 培养，将每个培养孔的菌落培养到布鲁氏菌琼脂平板上。对每个平板进行菌落计数：结果显示，第三次注射联合疫苗的滴度最高，为 1 64，疫苗有效率为 87.71%：总之，这项研究结果表明，流产鲍鱼中的 LPS 和 OMP 可提供可接受的免疫力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Avicenna journal of medical biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

2.90

自引率

0.00%

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