Prevalence and classification of meniscal calcifications in the human knee

IF 7.2 2区医学 Q1 ORTHOPEDICS

Osteoarthritis and Cartilage Pub Date : 2024-08-06 DOI:10.1016/j.joca.2024.07.013

Bijay Ratna Shakya , Ville-Pauli Karjalainen , Iida Hellberg , Mikko A.J. Finnilä , Khaled Elkhouly , Amanda Sjögren , Aleksandra Turkiewicz , Patrik Önnerfjord , Velocity Hughes , Jon Tjörnstrand , Martin Englund , Simo Saarakkala

{"title":"Prevalence and classification of meniscal calcifications in the human knee","authors":"Bijay Ratna Shakya , Ville-Pauli Karjalainen , Iida Hellberg , Mikko A.J. Finnilä , Khaled Elkhouly , Amanda Sjögren , Aleksandra Turkiewicz , Patrik Önnerfjord , Velocity Hughes , Jon Tjörnstrand , Martin Englund , Simo Saarakkala","doi":"10.1016/j.joca.2024.07.013","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the occurrence of meniscal calcifications in individuals with and without knee osteoarthritis (OA). Additionally, we aim to identify the specific types of calcifications: basic calcium phosphate (BCP) and calcium pyrophosphate dihydrate (CPP).</div></div><div><h3>Method</h3><div>We analyzed 82 meniscal posterior horn samples (medial and lateral) collected from 41 human subjects. Among them, 20 individuals underwent total knee replacement due to medial compartment OA, while 21 deceased donors had no known knee OA. The assessment of meniscal calcifications and Pauli’s histopathological scoring was conducted using histological sections. Furthermore, adjacent sections underwent measurement using Raman spectroscopy to characterize BCP and CPP calcifications based on their distinct spectral fingerprints.</div></div><div><h3>Results</h3><div>All OA individuals exhibited calcifications in at least one meniscus, compared to 9.5% (95%CI 1%, 30%) of donors. Among 35 OA menisci with calcifications, 28(80%) had BCP, 5(14%) had CPP and 2(6%) had both types. In 4 donor menisci, 3(75%) had CPP while 1(25%) had both types. We estimated the association between Pauli score and presence of BCP in OA individuals, yielding an odds ratio of 2.1 (95%CI 0.8, 5.3) per 1 Pauli score. The association between Pauli score and presence of CPP (in whole study sample) seemed weaker, with odds ratio of 1.3 (95%CI 1.1, 1.7).</div></div><div><h3>Conclusion</h3><div>The presence of BCP was predominant in menisci of OA individuals, whereas CPP exhibited similar prevalence in individuals with and without OA. The formation of BCP crystals in menisci may represent an important and specific characteristic of OA disease process that warrants further attention.</div></div>","PeriodicalId":19654,"journal":{"name":"Osteoarthritis and Cartilage","volume":"32 11","pages":"Pages 1443-1451"},"PeriodicalIF":7.2000,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Osteoarthritis and Cartilage","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1063458424013190","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ORTHOPEDICS","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

To investigate the occurrence of meniscal calcifications in individuals with and without knee osteoarthritis (OA). Additionally, we aim to identify the specific types of calcifications: basic calcium phosphate (BCP) and calcium pyrophosphate dihydrate (CPP).

Method

We analyzed 82 meniscal posterior horn samples (medial and lateral) collected from 41 human subjects. Among them, 20 individuals underwent total knee replacement due to medial compartment OA, while 21 deceased donors had no known knee OA. The assessment of meniscal calcifications and Pauli’s histopathological scoring was conducted using histological sections. Furthermore, adjacent sections underwent measurement using Raman spectroscopy to characterize BCP and CPP calcifications based on their distinct spectral fingerprints.

Results

All OA individuals exhibited calcifications in at least one meniscus, compared to 9.5% (95%CI 1%, 30%) of donors. Among 35 OA menisci with calcifications, 28(80%) had BCP, 5(14%) had CPP and 2(6%) had both types. In 4 donor menisci, 3(75%) had CPP while 1(25%) had both types. We estimated the association between Pauli score and presence of BCP in OA individuals, yielding an odds ratio of 2.1 (95%CI 0.8, 5.3) per 1 Pauli score. The association between Pauli score and presence of CPP (in whole study sample) seemed weaker, with odds ratio of 1.3 (95%CI 1.1, 1.7).

Conclusion

The presence of BCP was predominant in menisci of OA individuals, whereas CPP exhibited similar prevalence in individuals with and without OA. The formation of BCP crystals in menisci may represent an important and specific characteristic of OA disease process that warrants further attention.

查看原文本刊更多论文

人类膝关节半月板钙化的发生率和分类。

目的研究膝关节骨性关节炎（OA）患者和非膝关节骨性关节炎患者半月板钙化的发生率。此外，我们还旨在确定钙化的具体类型：基本磷酸钙（BCP）和二水焦磷酸钙（CPP）：我们分析了从 41 名受试者身上采集的 82 个半月板后角样本（内侧和外侧）。其中，20 人因内侧室 OA 而接受了全膝关节置换术，21 名已故捐献者没有已知的膝关节 OA。通过组织切片对半月板钙化和保利组织病理学评分进行了评估。此外，还使用拉曼光谱对相邻切片进行测量，根据其不同的光谱指纹来确定BCP和CPP钙化的特征：所有 OA 患者至少有一个半月板出现钙化，而捐献者的比例为 9.5%（95%CI 1%，30%）。在 35 个有钙化的 OA 半月板中，28 个（80%）有 BCP，5 个（14%）有 CPP，2 个（6%）有两种类型。在 4 个供体的半月板中，3 个（75%）有 CPP，1 个（25%）两种类型都有。我们估算了保利评分与 OA 患者是否存在 BCP 之间的关系，结果显示，每 1 个保利评分的几率比为 2.1（95%CI 0.8，5.3）。在整个研究样本中，保利得分与是否存在CPP之间的关联似乎较弱，几率比为1.3（95%CI 1.1，1.7）：结论：BCP主要存在于OA患者的半月板中，而CPP在OA患者和非OA患者中的发生率相似。BCP晶体在半月板中的形成可能代表了OA疾病过程中的一个重要而特殊的特征，值得进一步关注。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Osteoarthritis and Cartilage 医学-风湿病学

CiteScore

11.70

自引率

7.10%

发文量

802

审稿时长

52 days

期刊介绍： Osteoarthritis and Cartilage is the official journal of the Osteoarthritis Research Society International. It is an international, multidisciplinary journal that disseminates information for the many kinds of specialists and practitioners concerned with osteoarthritis.