Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024.

IF 9.9 2区医学 Q1 INFECTIOUS DISEASES

Eurosurveillance Pub Date : 2024-08-01 DOI:10.2807/1560-7917.ES.2024.29.32.2400486

Leonard Schuele, Leandre Murhula Masirika, Jean Claude Udahemuka, Freddy Belesi Siangoli, Justin Bengehya Mbiribindi, Pacifique Ndishimye, Frank M Aarestrup, Marion Koopmans, Bas B Oude Munnink, Richard Molenkamp

引用次数: 0

Abstract

Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.

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实时 PCR 检测新型 Ib 支猴痘病毒，2023 年 9 月至 2024 年 5 月。

猴痘病毒（MPXV）是一种新出现的人畜共患病原体，流行病学复杂，需要快速诊断并区分支系和亚支系。新出现的 Ib 支缺乏美国疾病控制和预防中心 I 支特异性检测中使用的基因组区域。我们报告了一种用于特异性检测支系 Ib 的 MPXV 实时 PCR。该检测方法在 92 份样本中表现出良好的灵敏度和特异性，可与其他基于 TaqMan 的检测方法一起用于检测 MPXV 并区分支系和亚支系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Eurosurveillance INFECTIOUS DISEASES-

CiteScore

32.70

自引率

2.10%

发文量

430

审稿时长

3-8 weeks

期刊介绍： Eurosurveillance is a European peer-reviewed journal focusing on the epidemiology, surveillance, prevention, and control of communicable diseases relevant to Europe.It is a weekly online journal, with 50 issues per year published on Thursdays. The journal includes short rapid communications, in-depth research articles, surveillance reports, reviews, and perspective papers. It excels in timely publication of authoritative papers on ongoing outbreaks or other public health events. Under special circumstances when current events need to be urgently communicated to readers for rapid public health action, e-alerts can be released outside of the regular publishing schedule. Additionally, topical compilations and special issues may be provided in PDF format.