Tools and methods for cell ablation and cell inhibition in Caenorhabditis elegans.

IF 3.3 3区生物学 Q2 GENETICS & HEREDITY

Genetics Pub Date : 2024-08-07 DOI:10.1093/genetics/iyae119

Dennis Rentsch, Amelie Bergs, Jiajie Shao, Nora Elvers, Christiane Ruse, Marius Seidenthal, Ichiro Aoki, Alexander Gottschalk

引用次数: 0

Abstract

To understand the function of cells such as neurons within an organism, it can be instrumental to inhibit cellular function, or to remove the cell (type) from the organism, and thus to observe the consequences on organismic and/or circuit function and animal behavior. A range of approaches and tools were developed and used over the past few decades that act either constitutively or acutely and reversibly, in systemic or local fashion. These approaches make use of either drugs or genetically encoded tools. Also, there are acutely acting inhibitory tools that require an exogenous trigger like light. Here, we give an overview of such methods developed and used in the nematode Caenorhabditis elegans.

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在秀丽隐杆线虫中进行细胞消融和细胞抑制的工具和方法。

要了解生物体内神经元等细胞的功能，抑制细胞功能或将细胞（类型）从生物体中移除，从而观察其对生物体和/或回路功能以及动物行为的影响是非常有用的。在过去的几十年里，人们开发并使用了一系列方法和工具，这些方法和工具以全身或局部的方式发挥组成性或急性和可逆性作用。这些方法利用药物或基因编码工具。此外，还有一些需要外源触发（如光）的急性抑制工具。在此，我们将概述在线虫中开发和使用的此类方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics GENETICS & HEREDITY-

CiteScore

6.90

自引率

6.10%

发文量

177

审稿时长

1.5 months

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.