Ameliorating and Therapeutic Impact of Curcumin Nanoparticles Against Aluminum Oxide Nanoparticles Induced Kidney Toxicity, DNA Damage, Oxidative Stress, PCNA and TNFα Alteration in Male Rats

Abstract

Aluminum oxide nanoparticles (Al₂O₃ NPs) are among the most extensively utilized nanoparticles in nanotechnology and that have negative impacts on the environment. Therefore, the intention of this work is to investigate the protective and therapeutic effects of curcumin in nanoform (Cur NPs) against Al₂O₃ NPs induced kidney toxicity, oxidative stress, DNA damage, and changes in necrosis factor alpha (TNFα) and proliferating cell nuclear antigen (PCNA) expressions in male rats. Fifty healthy adult male were divided into five groups [G1, control; G2, received 50 mg/kg/day for 4 weeks of Cur NPs orally; G3, received 6 mg/kg BW orally for 4 weeks of Al₂O₃ NPs; G4, (Cur NPs + Al₂O₃ NPs) received Cur NPs and Al₂O₃ NPs at a dose similar to G2 and G3, respectively for 4 weeks; G5, (Al₂O₃ NPs + Cur NPs) received Al₂O₃ NPs at a dose similar to G3 for 4 weeks then received Cur NPs at a dose similar to G2 for another 4 weeks]. Current results revealed that Al₂O₃ NPs induced a significant elevation in serum urea, creatinine, chloride, calcium, kidney malondialdehyde (MDA), DNA damage, injury, TNFα and PCNA expressions and a significant depletion in serum potassium, kidney superoxide dismutase (SOD), glutathione (GSH) as compared to control. On the other hand, treatments of Al₂O₃ NPs with Cur NPs induced modulation in all altered parameters and improved kidney functions and structure, with best results for the Al₂O₃ NPs + Cur NPs than Cur NPs + Al₂O₃ NPs. In conclusion, Cur NPs has the capacity to mitigate the renal toxicity induced by Al₂O₃ NPs in male albino rats.