Idebenone protects the kidneys of rats with renovascular arterial hypertension by inhibiting oxidative stress and apoptosis.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-28 DOI:10.14715/cmb/2024.70.7.26

Jinsen He, Lixin Huang, Linqi Lu

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引用次数: 0

Abstract

Here, the protective effect of antioxidant Idebenone (IDB) on renovascular hypertension was studied. The two-kidney one-clip (2K-1C) model of renal hypertension was established. The rats were divided into 3 groups: sham-operation group, 2K-1C renal hypertensive rats' model group and model treated with IDB group. The mean arterial blood pressure (MBP) of rats was measured and pathological condition of kidney was observed by H&E staining. The change of renal damage biomarkers (Cre, BUN, urine proteins), inflammatory factors (IL-6, IL-1β and TNF-α), oxidative stress ratio and key factors (MDA, SOD and CAT) were assessed by kits. The apoptosis key proteins (BAD, BAX, Caspase9, GSK-3β) were detected via Western blot. The 2K-1C model of renal hypertension was established. IDB reduced the MBP, Cre, BUN, urine proteins and improved the pathological condition of 2K-1C kidney. IDB restrained the inflammation factors (IL-6, IL-1β and TNF-α) and oxidative stress in kidney of renal hypertensive rats' model. Besides, IDB suppressed the expression of apoptosis key factors (BAD, BAX, Caspase9, GSK-3β) in kidney of renal hypertensive rats' model. IDB protects the kidneys of rats with renovascular arterial hypertension by inhibiting inflammation, oxidative stress, and apoptosis. These findings might provide medication guidance for IDB in renovascular arterial hypertension.

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艾地苯醌可通过抑制氧化应激和细胞凋亡来保护新血管性动脉高血压大鼠的肾脏。

本文研究了抗氧化剂艾地苯醌（IDB）对新血管性高血压的保护作用。建立双肾单夹（2K-1C）肾性高血压模型。大鼠分为 3 组：假手术组、2K-1C 肾性高血压模型组和 IDB 治疗模型组。测量大鼠的平均动脉血压（MBP），并通过 H&E 染色观察肾脏的病理状况。用试剂盒评估肾损伤生物标志物（Cre、BUN、尿蛋白）、炎症因子（IL-6、IL-1β和TNF-α）、氧化应激比率和关键因子（MDA、SOD和CAT）的变化。通过 Western 印迹检测凋亡关键蛋白（BAD、BAX、Caspase9、GSK-3β）。建立了 2K-1C 肾性高血压模型。IDB降低了MBP、Cre、BUN和尿蛋白，改善了2K-1C肾脏的病理状况。IDB抑制了肾性高血压大鼠肾脏中的炎症因子（IL-6、IL-1β和TNF-α）和氧化应激。此外，IDB 还能抑制肾脏凋亡关键因子（BAD、BAX、Caspase9、GSK-3β）的表达。IDB通过抑制炎症、氧化应激和细胞凋亡来保护肾动脉高血压大鼠的肾脏。这些发现可为IDB治疗新血管性动脉高血压提供药物指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.