LncRNA linc01105 inhibits gastric cancer growth and metastasis by regulating the miR-650/TCEA3 axis

IF 2.1 4区医学 Q3 TOXICOLOGY

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2024-08-01 DOI:10.1615/jenvironpatholtoxicoloncol.2024054112

Qiang Zhang, Hui Mi, Huimei Cai, Chenhui Li, Zhou Wu, Jianfang Zhang

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引用次数: 0

Abstract

Background: Gastric cancer (GC) is a widespread cancerous disease with an unfavorable prognosis, linc01105 appears to have a significant connection with the development of GC, but the precise regulatory mechanism remains obscure. Methods: RT-qPCR was utilized to determine the expression of linc01105, miR-650, and TCEA3. Proliferative capacity of GC cells was measured by CCK8. Cell apoptosis rates were analyzed via flow cytometry assessment. The invasive capability was assessed through the transwell experiment. The targeted regulation of linc01105, miR-650, and TCEA3 was validated through dual luciferase reporter gene assay. Result: Low expression of linc01105, high expression of miR-650, and low expression of TCEA3 were observed in GC tumor tissues and cell lines. High expression of linc01105 was correlated with a positive prognosis in GC patients. Linc01105 affected GC cell function by miR-650/TCEA3 axis. Conclusion: This study suggested that linc01105 may possess inhibitory functions towards GC. In addition, linc01105 may be a prognostic marker for GC.

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LncRNA linc01105通过调节miR-650/TCEA3轴抑制胃癌的生长和转移

背景：胃癌（GC）是一种广泛存在且预后不良的癌症，linc01105似乎与GC的发生发展有重要关系，但其确切的调控机制仍不清楚：方法：利用 RT-qPCR 检测 linc01105、miR-650 和 TCEA3 的表达。用 CCK8 测定 GC 细胞的增殖能力。细胞凋亡率通过流式细胞术评估进行分析。侵袭能力通过透孔实验进行评估。通过双荧光素酶报告基因实验验证了 linc01105、miR-650 和 TCEA3 的靶向调控作用：结果：在 GC 肿瘤组织和细胞系中观察到 linc01105 低表达、miR-650 高表达和 TCEA3 低表达。Linc01105 的高表达与 GC 患者的预后呈正相关。Linc01105通过miR-650/TCEA3轴影响GC细胞功能：这项研究表明，linc01105可能对GC具有抑制作用。结论：这项研究表明，linc01105可能对GC具有抑制作用，此外，linc01105可能是GC的预后标志物。

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来源期刊

Journal of Environmental Pathology Toxicology and Oncology 医学-毒理学

CiteScore

3.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Environmental Pathology, Toxicology and Oncology publishes original research and reviews of factors and conditions that affect human and animal carcinogensis. Scientists in various fields of biological research, such as toxicologists, chemists, immunologists, pharmacologists, oncologists, pneumologists, and industrial technologists, will find this journal useful in their research on the interface between the environment, humans, and animals.