Anion effects on the structure and stability of 3D domain-swapped cytochrome c dimer

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-08-02 DOI:10.1093/chemle/upae150

Kodai Fujiwara, Michiko Ryuzaki, Masaru Yamanaka, Tsuyoshi Mashima, Tomonori Saotome, Shun-ichi Kidokoro, Shun Hirota

引用次数: 0

Abstract

The structure and stability of proteins are affected by ions. We have previously reported that cytochrome c can form a 3D domain-swapped dimer. The structure of the cytochrome c domain-swapped dimer becomes more compact in the presence of kosmotropic anions, whereas the thermal stability of the dimer decreased with addition of chaotropic anions based on the Hofmeister series. Dimers were formed by incubating cytochrome c at 50 °C in the presence of citrate ions.

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阴离子对三维结构域互换的细胞色素 c 二聚体的结构和稳定性的影响

蛋白质的结构和稳定性受到离子的影响。我们以前曾报道过细胞色素 c 可以形成三维结构域互换二聚体。细胞色素 c 结构域互换二聚体的结构在各向同性阴离子的存在下变得更加紧凑，而二聚体的热稳定性则随着基于霍夫迈斯特系列的各向混沌阴离子的加入而降低。将细胞色素 c 在柠檬酸根离子存在下于 50 °C孵育可形成二聚体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.