Use of Adjuvant Compositions Based on Squalene Ensures Induction of Neutralizing Antibodies against SARS-CoV-2.

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2024-06-01 Epub Date: 2024-08-02 DOI:10.1007/s10517-024-06160-6

E A Volosnikova, N V Volkova, V V Ermolaev, M B Borgoyakova, V S Nesmeyanova, A V Zaykovskaya, O V Pyankov, B N Zaitsev, S V Belenkaya, A A Isaeva, D N Shcherbakov

引用次数: 0

Abstract

Squalene-based adjuvant compositions that can provide effective induction of specific humoral immune response have been developed. Recombinant receptor-binding domain (RBD) of surface S-protein of SARS-CoV-2 was used to evaluate the properties of the composition. Immunization of mice with the developed squalene-based compositions in combination with RBD allows obtaining high titers of specific antibodies: from 10⁵ to 2×10⁶. The blood sera from immunized mice exhibit neutralizing activity against SARS-CoV-2 Delta variant (B.1.617.2) with a titer up to 1:2000.

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使用基于角鲨烯的佐剂复合物可确保诱导针对 SARS-CoV-2 的中和抗体。

现已开发出能有效诱导特异性体液免疫反应的角鲨烯基佐剂组合物。SARS-CoV-2表面S蛋白的重组受体结合域（RBD）被用来评估组合物的特性。用开发的角鲨烯基组合物与 RBD 结合对小鼠进行免疫，可获得高滴度的特异性抗体：从 105 到 2×106。免疫小鼠的血清对 SARS-CoV-2 Delta 变体（B.1.617.2）具有中和活性，滴度高达 1:2000。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.