An Enzyme Free Electrochemical Assay Utilizing Graphene Oxide Sheets Decorated with Silver Nanoparticles for the Identification of Pseudomonas aeruginosa
IF 2.1 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
N. Krithiga, M. Vijayan, V. S. Vasantha, A. Jayachitra
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引用次数: 0
Abstract
This work describes the chronoamperometry deposition of Chitosan/silver nanocomposite and applied as platform for the development of immunoassay for the detection of Pseudomonas aeruginosa. The film was characterized by UV (Ultra visible spectroscopy), FTIR (Fourier transform infra red), Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray spectroscopy (EDAX) and electrochemical detection methods. The electrochemical detection of Pseudomonas aeruginosa was done based on the Anti Ps tagged with silver nanoparticles decorated graphene oxide sheets which in turn binds to the antigen and the response was observed using Hydrogen peroxide (H2O2) in PB (Phosphate Buffer) electrolyte. From the results, the sensitivity range is from 101 to 107 CFU/mL and LOD is calculated as 9.36 CFU/ml. The developed immunosensor also have high stability, reproducibility and reusability.
期刊介绍：
Indian Journal of Microbiology is the official organ of the Association of Microbiologists of India (AMI). It publishes full-length papers, short communication reviews and mini reviews on all aspects of microbiological research, published quarterly (March, June, September and December). Areas of special interest include agricultural, food, environmental, industrial, medical, pharmaceutical, veterinary and molecular microbiology.
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