Comparison of in-vitro orchid media solutions for Prosthechea cochleata (L.) W.E. Higgins vars. diandra and triandra

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Lauren G. Bartel, Jason Downing
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Abstract

Prosthechea cochleata or clamshell orchid is recognized as a species of both conservation and commercial importance. It has long been prized by orchid breeders and growers for its unique flower and hardy disposition. The commercial market has failed to meet the demand for this species since it is still targeted for illegal collection in the wild. This study examines the effectiveness of the banana powder on two variants (var. diandra and var. triandra) with disparate home ranges and levels of genetic diversity using two commercially available agar-based media (PhytoTechnology P668 and P748). Undifferentiated protocorms of the two variants were monitored for shoot growth and differentiation across a period of 156 days. The growth media with 3% banana powder supplement (P748) was more effective in initiating shoot formation in both P. cochleata variants compared to control media (P668) (F = 27.15, p < 0.001, df = 81). The diandrous variant grown with banana showed the highest mean shoot count (28.67 shoots/flask) at the end of the monitoring period. Results suggest that banana supplement is an effective source of plant growth regulators and organic nutrients necessary to promote shoot formation and seedling development in this species. This research is important for the conservation of P. cochleata as it identifies an effective and cost-efficient method for in vitro propagation, which can aid in increasing the ex-situ and in situ populations of this threatened species. Additionally, the findings have implications for commercial orchid production, as it can potentially improve the cultivation and yield of this species for economic purposes.

Abstract Image

Prosthechea cochleata (L.) W.E. Higgins vars. diandra 和 triandra 的体外兰花培养基溶液比较
蛤壳兰(Prosthechea cochleata)被认为是一种具有重要保护价值和商业价值的兰花。长期以来,它一直因其独特的花朵和耐寒性而受到兰花育种者和种植者的青睐。商业市场未能满足对该物种的需求,因为它仍然是野外非法采集的目标。本研究使用两种市售的琼脂培养基(PhytoTechnology P668 和 P748),考察了香蕉粉对两个变种(var. diandra 和 var.)对两个变种的未分化原球茎进行了为期 156 天的生长和分化监测。与对照培养基(P668)相比,添加 3% 香蕉粉的培养基(P748)对两个蜗牛变种的新芽形成更有效(F = 27.15,p < 0.001,df = 81)。在监测期结束时,用香蕉栽培的无柄变种显示出最高的平均芽数(28.67 个芽/瓶)。结果表明,香蕉补充物是植物生长调节剂和有机养分的有效来源,可促进该物种的嫩枝形成和幼苗发育。这项研究对保护鹅掌楸具有重要意义,因为它确定了一种有效且成本效益高的体外繁殖方法,有助于增加这一濒危物种的原生境和非原生境种群数量。此外,研究结果对商业兰花生产也有影响,因为它有可能提高该物种的栽培和产量,从而达到经济目的。
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来源期刊
Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物
CiteScore
5.40
自引率
13.30%
发文量
203
审稿时长
3.3 months
期刊介绍: This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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