Resolvin D1 (Rvd1) Attenuates In Vitro LPS-Stimulated Inflammation Through Downregulation of miR-155, miR -146, miR -148 and Krupple Like Factor 5.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jabbar Amin Mohedin, Alireza Rezaiemanesh, Soheila Asadi, Maryam Haddadi, Bahroz Abdul Ahmed, Ali Gorgin Karaji, Farhad Salari
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引用次数: 0

Abstract

Background: Chronic inflammation is associated with many inflammatory diseases. Specialized pro-resolving mediators (SPMs) are well known for their crucial role in promoting the resolution phase of inflammation and restoring tissue homeostasis. Resolvin D1 (RvD1) is an endogenous omega-3-derived lipid mediator with pro-resolving activity. This study aimed to evaluate the effect of Resolvin D1 (RvD1) on some inflammatory miRNAs (mir-155-5p, miR146a-5p and miR148-3p) and Krüppel-like factors 5 (KLF5) in an LPS-stimulated THP-1 preclinical model of inflammation.

Methods: PMA-differentiated THP-1 cells (macrophages) were pre-incubated with or without various concentrations of RvD1 (10, 50, or 100 nM) for 2 h prior to stimulation by 1 μg/ml LPS. Un-stimulated PMA-differentiated THP-1 cells were as the control group. Then, the expression levels of target genes were evaluated by real-time PCR.

Results: Compared with untreated macrophages, stimulation with 1 µg/ml LPS increased mRNA expression levels of TNF-α, KLF5, miR-155-5p, miR-146-5p, and miR-148a-3p. When the cells were exposed to various concentrations (10, 50 and 100 nM) of RvD1 for 2 h prior to LPS stimulation, the TNF-α, KLF5, miR-155-5p, miR-146-5p, and miR-148a-3p mRNA expression levels were significantly downregulated in a dose-dependent manner, compared to the LPS group.

Conclusions: The results demonstrate that RvD1 can attenuate inflammatory response in LPS-stimulated macrophages. Our data also showed that RvD1 may exert anti-inflammatory effects by inhibiting miR-155-5p, miR-146a-5p, and miR-148-3p.

Resolvin D1 (Rvd1) 通过下调 miR-155、miR -146、miR -148 和 Krupple Like Factor 5,减轻体外 LPS 刺激的炎症反应。
背景:慢性炎症与许多炎症性疾病有关:慢性炎症与许多炎症性疾病有关。众所周知,特化的促进炎症消解介质(SPMs)在促进炎症消解阶段和恢复组织稳态方面发挥着至关重要的作用。Resolvin D1(RvD1)是一种内源性欧米伽-3衍生脂质介质,具有促进消炎的活性。本研究旨在评估在LPS刺激的THP-1临床前炎症模型中,Resolvin D1(RvD1)对一些炎症miRNA(mir-155-5p、miR146a-5p和miR148-3p)和Krüppel样因子5(KLF5)的影响:在 1 μg/ml LPS 刺激前,将 PMA 分化的 THP-1 细胞(巨噬细胞)与不同浓度的 RvD1(10、50 或 100 nM)预孵育 2 小时。未受刺激的 PMA 分化 THP-1 细胞为对照组。然后,通过实时 PCR 评估目标基因的表达水平:结果:与未处理的巨噬细胞相比,1 µg/ml LPS 刺激会增加 TNF-α、KLF5、miR-155-5p、miR-146-5p 和 miR-148a-3p 的 mRNA 表达水平。当细胞在LPS刺激前暴露于不同浓度(10、50和100 nM)的RvD1 2小时后,与LPS组相比,TNF-α、KLF5、miR-155-5p、miR-146-5p和miR-148a-3p mRNA表达水平呈剂量依赖性显著下调:结果表明,RvD1 可减轻 LPS 刺激的巨噬细胞的炎症反应。我们的数据还表明,RvD1 可通过抑制 miR-155-5p、miR-146a-5p 和 miR-148-3p 发挥抗炎作用。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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