Ocimum basilicum L. (basil) presents pro-apoptotic activity in an Ehrlich's experimental tumor murine model.

Acta cirurgica brasileira Pub Date : 2024-07-29 eCollection Date: 2024-01-01 DOI:10.1590/acb393924

Phelipe Gabriel Dos Santos Sant'Ana, William Gustavo Lima, Gabriela Francine Martins Lopes, Sabrina Elisa de Oliveira, Guilherme Augusto Ferreira da Costa, Luciana Alves Rodrigues Dos Santos Lima, Elisângela Elduina Ferreira, Ivan Carlos Dos Santos, Laila Cristina Moreira Damázio, Rosy Iara Maciel Azambuja Ribeiro, Flávia Carmo Horta Pinto

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Abstract

Purpose: This study aimed to evaluate the therapeutic effect of an ethanol extract of Ocimum basilicum L. (EEOb) aerial parts against Ehrlich's experimental tumor (EET) in mice.

Methods: Swiss mice were divided into two groups (control and treated; n = 6). On day 21, all mice were inoculated subcutaneously with 2 × 106 (0.05 mL) EET cells in the left paw for solid tumor development. This study lasted 28 days. Treatment began 24 hours after inoculation with EET. Measurements of dorsoplantar thickness were used to assess tumor growth. The paw pad was collected for histopathological analysis and stained using the argyrophilic nucleolar organizing regions (AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen, Bcl-2 and Bax.

Results: The treatment of animals with EEOb at 100 mg/kg intraperitoneally was able to reduce the growth (Control = 3.7 ± 0.1 mm vs. EEOb = 5.7 ± 0.2 mm) and the number of AgNORs of solid Ehrlich tumor. The antitumor effect of EEOb was associated with the induction of apoptosis of tumoral cell, as suggested by the reduction of the content of Bcl-2 induced by extract.

Conclusions: The study demonstrated that daily administration of EEOb is able to reduce the growth of EET by induce apoptosis of tumoral cells.

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罗勒（Ocimum basilicum L.）在艾氏实验性肿瘤小鼠模型中具有促进凋亡的活性。

目的：本研究旨在评估欧加木紫苏叶乙醇提取物（EEOb）对小鼠艾氏实验性肿瘤（EET）的治疗效果：方法：瑞士小鼠分为两组（对照组和治疗组；n = 6）。第 21 天，所有小鼠左爪皮下注射 2 × 106（0.05 mL）个 EET 细胞，进行实体瘤培养。这项研究持续了 28 天。接种 EET 24 小时后开始治疗。背跖厚度测量用于评估肿瘤生长情况。收集爪垫进行组织病理学分析，并使用嗜曙光细胞核组织区（AgNOR）技术和增殖细胞核抗原、Bcl-2和Bax的免疫组化技术进行染色：结果：以100毫克/千克的剂量腹腔注射EEOb可减少艾氏实体瘤的生长（对照组=3.7±0.1毫米，EEOb=5.7±0.2毫米）和AgNOR的数量。EEOb的抗肿瘤作用与诱导肿瘤细胞凋亡有关，提取物诱导的Bcl-2含量降低也说明了这一点：研究表明，每天服用EEOb能够通过诱导肿瘤细胞凋亡来减少EET的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta cirurgica brasileira

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