The Correlation between Lung Ultrasound and Pathology in Rat Model of Monocrotaline-Induced Pulmonary Hypertension.

IF 2.1 4区 医学 Q3 RESPIRATORY SYSTEM
Canadian respiratory journal Pub Date : 2024-07-23 eCollection Date: 2024-01-01 DOI:10.1155/2024/6619471
Yan-Fen Zhong, Bin-Bin Liang, Xiao-Feng Zhang, Ji-Wu
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Abstract

Pulmonary hypertension (PH) is a progressive and complex pulmonary vascular disease with poor prognosis. The aim of this study was to provide a new understanding of the lung pathology of disease and a noninvasive method in monitoring the establishment of animal models for basic and clinical studies of PH, indeed to explore clinical application value of lung ultrasound for patients with PH. Totally 32 male SD rats were randomly divided into control group, MCT (monocrotaline) group, PDTC (pyrrolidine dithiocarbamate) group, and NS (normal saline) group. Rats in the MCT group, PDTC group, and NS group received single intraperitoneal injection of MCT, while the control group received the same dose of NS. Then, PDTC group and NS group received PDTC and NS daily for treatment at the end of the model. Each group received lung ultrasound examination and measurement of pulmonary arterial pressure (PAP). Then, the rats were sacrificed to take the lung specimens to being observed. The ultrasound and pathological results were analyzed with a semiquantitative score. With the pulmonary artery pressure increases, the MCT group had a higher pulmonary ultrasound score and pathological score compared with the control group (p < 0.05). After PDTC treatment, the pulmonary ultrasound score and the pathological score decline (p < 0.05). We investigated both lung ultrasound scores, and the pathological scores were positively correlated with mean pulmonary artery pressure (mPAP) (both r > 0.8, p < 0.0001). Moreover, lung ultrasound scores were positively correlated with pathological scores (r > 0.8, p < 0.0001). We elucidated lung ultrasound evaluation providing more evidence for the management of PH in the rat model. Moreover, lung ultrasound provided a noninvasive method in monitoring the establishment of animal models for basic and clinical studies of PH.

单克隆盐碱诱发肺动脉高压大鼠模型中肺部超声波与病理学之间的相关性
肺动脉高压(PH)是一种进展性、复杂的肺血管疾病,预后不良。本研究的目的是提供一种对肺部病理的新认识和一种无创的监测方法,以建立肺动脉高压基础和临床研究的动物模型,并探索肺部超声对肺动脉高压患者的临床应用价值。32只雄性SD大鼠被随机分为对照组、MCT(单克隆)组、PDTC(吡咯烷二硫代氨基甲酸盐)组和NS(生理盐水)组。MCT组、PDTC组和NS组的大鼠腹腔注射一次MCT,而对照组则注射相同剂量的NS。然后,PDTC 组和 NS 组在模型结束后每天接受 PDTC 和 NS 治疗。每组大鼠均接受肺部超声波检查和肺动脉压(PAP)测量。然后,大鼠被处死,取肺标本进行观察。对超声和病理结果进行半定量评分分析。与对照组相比,随着肺动脉压力的升高,MCT 组的肺部超声评分和病理评分更高(P < 0.05)。PDTC 治疗后,肺超声评分和病理评分下降(P < 0.05)。我们研究发现,肺部超声评分和病理评分均与平均肺动脉压(mPAP)呈正相关(r 均大于 0.8,p < 0.0001)。此外,肺部超声评分与病理评分呈正相关(r > 0.8,p < 0.0001)。我们阐明了肺部超声评估,为大鼠模型中 PH 的治疗提供了更多证据。此外,肺部超声还为建立 PH 的基础和临床研究动物模型提供了一种无创的监测方法。
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来源期刊
Canadian respiratory journal
Canadian respiratory journal 医学-呼吸系统
CiteScore
4.20
自引率
0.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Canadian Respiratory Journal is a peer-reviewed, Open Access journal that aims to provide a multidisciplinary forum for research in all areas of respiratory medicine. The journal publishes original research articles, review articles, and clinical studies related to asthma, allergy, COPD, non-invasive ventilation, therapeutic intervention, lung cancer, airway and lung infections, as well as any other respiratory diseases.
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