Formulation, characterization and evaluation of anti-breast cancer activity of 2-carene nanoemulsion; in silico, in vitro and in vivo study

IF 5.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Arabian Journal of Chemistry Pub Date : 2024-07-25 DOI:10.1016/j.arabjc.2024.105937

Iffat Nayila , Sumaira Sharif , Madeeha Shahzad Lodhi , Riaz Ullah , Amal Alotaibi , Tahir Maqbool , Saima Hameed

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Abstract

Natural phytocompounds extensively used in several therapeutic regimes, biomedical and pharmaceutical applications and their nanoparticles have become extremely essential in field of nanotechnology to treat various types of cancer. The main objective of this study demonstrates the antitumor activities of pure terpene compound 2-carene and its nanoemulsion against in vitro and in vivo breast cancer model. In silico and molecular dynamic simulation study was performed against breast cancer receptors to evaluate anticancer activity of 2-carene. Nanoemulsions were prepared by isolating monoterpenoid compound from Catharanthus roseus essential oil and anti-cancer effects of 2-carene mediated nanoemulsion were evaluated. Gas chromatography-mass spectrometry analysis explicated 2-carene was the active terpene compound and nuclear magnetic resonance spectroscopy was performed to confirm structural identification of this compound. Nanoemulsion was characterized by characteristic techniques of scanning electron microscopy, Fourier transform infrared spectroscopy, size determination and dynamic light scattering. Cytotoxicity, antioxidant activity, apoptosis and histopathological examination were used to identify bioactive qualities of prepared nanoemulsion formulation. Effective cytotoxicity of nanoemulsion was observed 54.31% against MDA-MB-231 cancer cell lines. Tumor features and apoptotic activity was also assessed and the results of study strongly revealed that bio-synthesized nanoemulsion show significant apoptotic and anti-tumor potential against DMBA induced breast cancer. The antioxidant enzymes and serum inflammatory markers were significantly improved by nanoemulsion treatment in cancer induced rat model. In histopathological evaluation, the nanoemulsion formulations markedly show signs of improvement in cancer induced tissues. Results demonstrated that 2-carene and its synthesized nanoemulsion exhibit remarkable anticancer potential against DMBA induced cancer.

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2-carene 纳米乳液的制备、表征和抗乳腺癌活性评估；硅学、体外和体内研究

天然植物化合物被广泛应用于多种治疗方法、生物医学和制药领域，其纳米颗粒也成为纳米技术领域治疗各种癌症的关键。本研究的主要目的是展示纯萜烯化合物 2-蒈烯及其纳米乳液对体外和体内乳腺癌模型的抗肿瘤活性。针对乳腺癌受体进行了硅学和分子动力学模拟研究，以评估 2-蒈烯的抗癌活性。通过从石竹属植物精油中分离单萜化合物制备了纳米乳液，并评估了 2-卡瑞纳介导的纳米乳液的抗癌效果。气相色谱-质谱分析表明 2-蒈烯是活性萜烯化合物，核磁共振光谱分析证实了该化合物的结构鉴定。纳米乳液通过扫描电子显微镜、傅立叶变换红外光谱、粒度测定和动态光散射等特征技术进行了表征。细胞毒性、抗氧化活性、细胞凋亡和组织病理学检查用于确定制备的纳米乳液配方的生物活性品质。纳米乳液对 MDA-MB-231 癌细胞株的有效细胞毒性为 54.31%。研究结果强烈显示，生物合成的纳米乳液对 DMBA 诱导的乳腺癌具有显著的凋亡和抗肿瘤潜力。在癌症诱导的大鼠模型中，纳米乳液的抗氧化酶和血清炎症指标均有明显改善。在组织病理学评估中，纳米乳液制剂对癌症诱导组织有明显的改善迹象。结果表明，2-蒈烯及其合成的纳米乳液对 DMBA 诱导的癌症具有显著的抗癌潜力。

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来源期刊

Arabian Journal of Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

10.80

自引率

3.30%

发文量

763

审稿时长

63 days

期刊介绍： The Arabian Journal of Chemistry is an English language, peer-reviewed scholarly publication in the area of chemistry. The Arabian Journal of Chemistry publishes original papers, reviews and short reports on, but not limited to: inorganic, physical, organic, analytical and biochemistry. The Arabian Journal of Chemistry is issued by the Arab Union of Chemists and is published by King Saud University together with the Saudi Chemical Society in collaboration with Elsevier and is edited by an international group of eminent researchers.