Trypsin inhibitors in seeds and pods of Phaseolus vulgaris/coccineus: A comparative study of shaking and ultrasonic extraction methods

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology Pub Date : 2024-07-25 DOI:10.1016/j.ejbt.2024.05.003

Hourieh Tavakoli Hasanaklou , Barbara Pipan , Vladimir Meglič , Nevena Nagl , Lovro Sinkovič

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引用次数: 0

Abstract

Background

Different methods for the extraction of trypsin inhibitors in beans (Phaseolus spp.) were investigated. Two randomised complete laboratory experiments were performed, one on the seeds and one on the pods. In the first, the seeds of common bean variety KIS Marcelijan, breeding line Ref_316 × 498 and runner bean variety Bonela were examined. In the second, the fresh pods of five common beans (three breeding lines, two varieties) were analysed. Four extraction methods were used, including ultrasonic-assisted extraction (UAE) for 15 and 30 min and shaking-assisted extraction for 60 and 180 min.

Results

The results showed a significant increase in trypsin inhibitor activity-related traits in UAE compared to shaking extraction, with the 15 min ultrasonic process showing better efficacy than the one with 30 min duration. In the seed experiment, the breeding line Ref_316 × 498 showed the highest Trypsin Units Inhibited (TUI) and TUI/mg sample after a 15 min UAE. In the pod experiment, the breeding line 228_4aa_ca also showed the highest TUI and TUI/mg sample after a 15 min extraction with UAE. These results underline the potential of UAE to maximise trypsin inhibitor content. In addition, remarkable correlations between TUI, TUI/mg sample and the percentage of trypsin inhibition (%TIn) were observed in both experiments.

Conclusions

These results provide valuable insights into the relationship between bean genetic resources, extraction methods and trypsin inhibitor content in bean pods and seeds and serve as a basis for refining extraction protocols. The study encourages further research on the practical implications of investigated protocols for breeding programmes and agricultural practices.

How to cite: Tavakoli Hasanaklou H, Pipan B, Meglič V, et al. Trypsin inhibitors in seeds and pods of Phaseolus vulgaris/coccineus: A comparative study of shaking and ultrasonic extraction methods. Electron J Biotechnol 2024;71. https://doi.org/10.1016/j.ejbt.2024.05.003.

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荚果中的胰蛋白酶抑制剂：振荡提取法和超声波提取法的比较研究

背景对豆类（Phaseolus spp.）中胰蛋白酶抑制剂的不同提取方法进行了研究。进行了两项随机完整实验室实验，一项针对种子，一项针对豆荚。第一项实验检测了普通豆品种 KIS Marcelijan、育种品系 Ref_316 × 498 和芸豆品种 Bonela 的种子。第二项研究分析了五种普通豆类（三个育种品系、两个品种）的新鲜豆荚。使用了四种提取方法，包括 15 分钟和 30 分钟的超声波辅助提取（UAE）以及 60 分钟和 180 分钟的振荡辅助提取。结果表明，与振荡提取相比，超声波辅助提取显著提高了胰蛋白酶抑制剂活性相关性状，其中 15 分钟的超声波提取比 30 分钟的提取效果更好。在种子实验中，育种品系 Ref_316 × 498 在 15 分钟超音波提取后显示出最高的胰蛋白酶抑制单位（TUI）和 TUI/mg样品。在豆荚实验中，用 UAE 提取 15 分钟后，育种品系 228_4aa_ca 也显示出最高的胰蛋白酶抑制单位（TUI）和胰蛋白酶抑制单位/mg 样品。这些结果凸显了 UAE 最大限度提高胰蛋白酶抑制剂含量的潜力。此外，在这两项实验中还观察到 TUI、TUI/mg 样品和胰蛋白酶抑制百分比（%TIn）之间存在明显的相关性。该研究鼓励进一步研究调查方案对育种计划和农业实践的实际影响：Tavakoli Hasanaklou H, Pipan B, Meglič V, et al：振荡法和超声波提取法的比较研究。Electron J Biotechnol 2024;71. https://doi.org/10.1016/j.ejbt.2024.05.003.

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来源期刊

Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering