BmfR, a novel GntR family regulator, regulates biofilm formation in marine-derived, Bacillus methylotrophicus B-9987

IF 6.1 1区 生物学 Q1 MICROBIOLOGY
Shanshan Xu , Zengzhi Liu , Pengfei Ren , Yang Liu , Fei Xiao , Wenli Li
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引用次数: 0

Abstract

Biofilms are common living states for microorganisms, allowing them to adapt to environmental changes. Numerous Bacillus strains can form complex biofilms that play crucial roles in biocontrol processes. However, our current understanding of the molecular mechanisms of biofilm formation in Bacillus is mainly based on studies of Bacillus subtilis. Knowledge regarding the biofilm formation of other Bacillus species remains limited. In this study, we identified a novel transcriptional regulator, BmfR, belonging to the GntR family, that regulates biofilm formation in marine-derived Bacillus methylotrophicus B-9987. We demonstrated that BmfR induces biofilm formation by activating the extracellular polysaccharide structural genes epsA-O and negatively regulating the matrix gene repressor, SinR; of note it positively affects the expression of the master regulator of sporulation, Spo0A. Furthermore, database mining for BmfR homologs has revealed their widespread distribution among many bacterial species, mainly Firmicutes and Proteobacteria. This study advances our understanding of the biofilm regulatory network of Bacillus strains, and provides a new target for exploiting and manipulating biofilm formation.

BmfR 是一种新型 GntR 家族调控因子,可调控海洋来源的甲基营养芽孢杆菌 B-9987 的生物膜形成
生物膜是微生物常见的生存状态,使其能够适应环境变化。许多芽孢杆菌菌株可以形成复杂的生物膜,在生物防治过程中发挥重要作用。然而,我们目前对枯草芽孢杆菌形成生物膜的分子机制的了解主要基于对枯草芽孢杆菌的研究。对其他芽孢杆菌生物膜形成的了解仍然有限。在本研究中,我们发现了一种属于 GntR 家族的新型转录调控因子 BmfR,它能调控海洋来源的甲基营养芽孢杆菌 B-9987 的生物膜形成。我们证明,BmfR 通过激活胞外多糖结构基因 epsA-O 和负向调节基质基因抑制因子 SinR 来诱导生物膜的形成;值得注意的是,它对孢子形成主调节因子 Spo0A 的表达有积极影响。此外,数据库对 BmfR 同源物的挖掘发现,它们广泛分布于许多细菌物种中,主要是固氮菌和变形菌。这项研究加深了我们对芽孢杆菌菌株生物膜调控网络的了解,并为利用和操纵生物膜的形成提供了一个新的目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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