Genetic Polymorphisms and Association of Growth Hormone Gene with Growth Traits in Beetal Goat

Indian Journal of Animal Research Pub Date : 2024-07-18 DOI:10.18805/ijar.b-5270

Surpreet Singh Dhillon, Dhirendra Kumar, Nazam Khan, D. Chakraborty, Kashif Dawood Khan, Vikas Mahajan

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Abstract

Background: This investigation was aimed to determine the association of growth hormone GH1 (A781G) and GH2 (A1575G) locus with growth traits in Beetal goats by using PCR-RFLP method. Methods: A total of 60 blood samples from Beetal goat were collected along with growth records (body weight in kg) at 0, 3, 6, 9 and 12 month of age maintained at Goat Dairy Farm Rajbagh, Jammu. DNA was extracted from blood samples by using DNA extraction kit. The target segment of GH 1 and 2 locus was amplified and digested with HaeIII restriction enzyme for scoring of genotypes. Result: The amplified PCR product obtained for GH1 (422 bp) and GH2 (116 bp) locus were digested with HaeIII restriction enzyme at 37°C for 15 min. The resultant digested products revealed three genotypes for GH1: AA (422 bp), BB (366 bp, 56 bp) and AB (422 bp, 366 bp and 56 bp) whereas, one genotype was observed for GH2 i.e., BB (88bp and 28bp). The genotypic frequencies in Beetal goat for GH1 were found to be 0.05 for AA genotype, 0.80 for AB genotype, 0.15 for BB genotype, whereas gene frequencies was 0.45 for A allele and 0.55 for B allele. GH2 locus was found to be monomorphic. The highly significant (P less than 0.01) Chi-square (χ²)-test value for GH1 locus showed that the population was not in HWE. BB genotype has a significantly higher (P less than 0.01) body weight followed by AA and AB genotypes at 9 and 12 month of age. Thus, GH1 locus can be used as a candidate gene to improve growth traits and selection of superior animals at an early age of 9 months for phenotypic selection programmes in Beetal goats.

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山羊生长激素基因的遗传多态性及其与生长性状的关系

研究背景本研究旨在利用 PCR-RFLP 方法测定生长激素 GH1 (A781G) 和 GH2 (A1575G) 基因座与贝他山羊生长性状的相关性。研究方法在查谟的拉杰巴格山羊奶牛场（Goat Dairy Farm Rajbagh, Jammu）收集了 60 份贝塔尔山羊的血液样本以及 0、3、6、9 和 12 月龄时的生长记录（体重，单位：千克）。使用 DNA 提取试剂盒从血液样本中提取 DNA。扩增 GH 1 和 2 基因座的目标片段，并用 HaeIII 限制性酶进行消化，以确定基因型。结果用 HaeIII 限制性酶在 37°C 下消化 GH1（422 bp）和 GH2（116 bp）基因座的扩增 PCR 产物 15 分钟。消化产物显示 GH1 有三种基因型：AA（422 bp）、BB（366 bp、56 bp）和 AB（422 bp、366 bp 和 56 bp），而 GH2 只有一种基因型，即 BB（88 bp 和 28 bp）。在 Beetal 山羊中，GH1 基因型频率为：AA 基因型 0.05、AB 基因型 0.80、BB 基因型 0.15，而基因频率为：A 等位基因 0.45、B 等位基因 0.55。GH2 基因座被发现是单态的。GH1位点的Chi-square（χ²）检验值极显著（P小于0.01），表明该群体不处于HWE状态。在 9 月龄和 12 月龄时，BB 基因型的体重明显较高（P 小于 0.01），其次是 AA 和 AB 基因型。因此，GH1基因位点可作为候选基因，用于改善贝他山羊的生长性状，并在9月龄早期进行表型选育，以选育出优良的动物。

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Indian Journal of Animal Research

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