Antibacterial Activity of Ethanolic Extract of Torch Ginger (Etlingera elatior) Towards Enterococcus faecalis: A Preliminary Research to the Alternative of Root Canal Irrigant of Primary Teeth

Abstract

Enterococcus faecalis is a notable pathogen found in the root canals of primary teeth with endodontic infections, often resistant to conventional root canal irrigation solutions, leading to treatment failure. Torch ginger or kecombrang (Etlingera elatior), a spice plant, is reported to contain bioactive compounds with antibacterial properties. This study investigates the antibacterial efficacy of ethanolic extract of torch ginger against Enterococcus faecalis, evaluating its potential as an alternative irrigant based on minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and effective concentration. Samples included Enterococcus faecalis ATCC® 29212™ and 70% ethanol extract of torch ginger, tested in eight concentrations (80%, 75%, 65%, 50%, 25%, 12.5%, 6.25%, and 3.125%) obtained through maceration, alongside two control groups (NaOCl and saline). The Kirby-Bauer disc diffusion method was employed to determine MIC, while the streaking method from MIC testing identified MBC. Data analysis for MIC and MBC values was conducted using the One-Way ANOVA parametric test (p < 0.05). Torch ginger extract demonstrated antibacterial activity against Enterococcus faecalis, with inhibition observed at a minimum concentration of 3.125% with average inhibition zone diameter of 9.63 ± 0.25 mm and reaching up to 80% concentration with average inhibition zone diameter of 15.03 ± 0.21 mm. The MIC was established at 3.125%, and the MBC among the tested concentrations was 80%. The study concludes that the ethanolic extract of torch ginger shows significant antibacterial activity against Enterococcus faecalis. An 80% concentration is identified as the most effective for inhibiting and killing the pathogen, suggesting its potential as an alternative root canal irrigant of primary teeth.