The Antifungal and Inhibitory Effects of Massoia Essential Oil and C10 Massoia Lactone on Mycotoxin Production in Fusarium graminearum KACC 41047

Q2 Agricultural and Biological Sciences

Agriculture Pub Date : 2024-07-24 DOI:10.3390/agriculture14081216

Jieun Lee, Sung-Eun Lee

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引用次数: 0

Abstract

In wheat and barley, Fusarium head blight is mainly caused by Fusarium graminearum, and its control is based on the agricultural practices of not leaving crop residues in the field, growing phytopathogenic fungi-resistant varieties, biological control, and chemical treatment, including using fungicides. Here, we investigated the antifungal and antimycotoxigenic activities of Massoia essential oil (MEO) and C10 Massoia lactone (C10) on Fusarium graminearum KACC 41047. Because DMSO, which was used as a solvent in this study, exhibited antifungal activity at 5% in a fungal growth medium, it was used in the antifungal and antimycotoxigenic experiments at 0.05%. Three assays were used to investigate the antifungal activities of MEO and C10, which exhibited potent antifungal activity in the agar dilution assay, with complete fungal growth inhibition at 100 mg/L. At 5–50 mg/L, MEO and C10 suppressed deoxynivalenol and 15-acetyl-deoxynivalenol production by >50% by downregulating the Tri10 gene, which expresses trichodiene synthase. MEO and C10 might be potent antifungal agents for F. graminearum control with less toxicological concerns because they are GRAS chemicals.

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麻黄精油和 C10 麻黄内酯对禾谷镰刀菌 KACC 41047 产生霉菌毒素的抗真菌和抑制作用

在小麦和大麦中，镰刀菌头枯病主要由禾谷镰刀菌引起，其防治主要依靠不在田间留下作物残茬、种植抗植物病原真菌的品种、生物防治和化学处理（包括使用杀菌剂）等农业措施。在此，我们研究了马苏叶精油（MEO）和 C10 马苏叶内酯（C10）对禾谷镰刀菌 KACC 41047 的抗真菌和抗霉菌毒素活性。由于本研究中用作溶剂的二甲基亚砜在真菌生长培养基中的抗真菌活性为 5%，因此在抗真菌和抗霉菌毒素实验中使用的二甲基亚砜的浓度为 0.05%。在琼脂稀释试验中，MEO 和 C10 具有很强的抗真菌活性，在 100 毫克/升时可完全抑制真菌生长。在 5-50 mg/L 的浓度下，MEO 和 C10 通过下调表达毛二烯合成酶的 Tri10 基因，可抑制脱氧雪腐镰刀菌烯醇和 15-乙酰脱氧雪腐镰刀菌烯醇的产生，抑制率大于 50%。由于 MEO 和 C10 属于 GRAS 化学品，因此它们可能是控制禾本科真菌的强效抗真菌剂，而且毒性问题较小。

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来源期刊

Agriculture Agricultural and Biological Sciences-Horticulture

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

11 weeks

期刊介绍： The Agriculture (Poľnohospodárstvo) is a peer-reviewed international journal that publishes mainly original research papers. The journal examines various aspects of research and is devoted to the publication of papers dealing with the following subjects: plant nutrition, protection, breeding, genetics and biotechnology, quality of plant products, grassland, mountain agriculture and environment, soil science and conservation, mechanization and economics of plant production and other spheres of plant science. Journal is published 4 times per year.