Research on the function of CG15382 by mutating Zelda binding region

Abstract

Zelda protein (Zld) is a transcription factor in drosophila. It binds on specific sequence to activate the expression of genes involved in the early development of drosophila, which hinted a method to study the function of these genes. Since the expression of genes can be regulated by Zld, mutation of Zld binding site can be used to silence target genes. Three base pairs were substituted by using CRISPR-Cas9 editing. The function of target genes in the development process can be studied by comparing wild type drosophila and the Zld absent mutant (Zld-). This paper will focus on CG15382 and discuss the design of the mutant drosophila.