Use of SATB2 and CDX2 Immunohistochemistry to Characterize and Diagnose Colorectal Cancer.

IF 1.3 4区 医学 Q3 ANATOMY & MORPHOLOGY
Shunsuke Kato, Akira Koshino, Jerzy Lasota, Masayuki Komura, Chengbo Wang, Masahide Ebi, Naotaka Ogasawara, Kazuhisa Kojima, Toyonori Tsuzuki, Kenji Kasai, Satoru Takahashi, Markku Miettinen, Kunio Kasugai, Shingo Inaguma
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引用次数: 0

Abstract

SATB2 has been reported to be highly specific for lower gastrointestinal tract tumors. On the basis of its ileum-colon conversion effects, which involve the activation of colonic genes in cooperation with CDX2 and HNF4A, we hypothesized that SATB2 and CDX2 might define the characteristics of colorectal cancers (CRCs). In the present study, the clinicopathologic and immunohistochemical characteristics of 269 CRCs were analyzed according to SATB2 and CDX2 expression. CRCs with SATB2- and/or CDX2- phenotypes showed associations with poorly differentiated histotypes ( P <0.00001), mucus production ( P =0.0019), and mismatch repair-deficient phenotypes ( P <0.00001). SATB2-/CDX2- CRCs were significantly associated with CK20-negativity, with or without CK7 expression ( P <0.00001), as well as with MUC5AC-positivity ( P <0.00001), and CD10-negativity ( P =0.00047). Negativity for SATB2 or CDX2 was associated with the expression of PD-L1 in both all CRC ( P <0.00001) and mismatch repair-proficient CRC ( P =0.000091). Multivariate Cox hazard regression analysis identified negativity for SATB2 and/or CDX2 as potential independent risk factors for patients with CRC. Regarding the diagnostic utility of SATB2, all of the 44 CRC metastases could be diagnosed as colorectal in origin if the immunohistochemical phenotypes (including CK7, CK20, and p53) of the primary lesions and patient history were considered. Among the other 684 tumors, we were unable to distinguish a case of CK7-/CK20+/CDX2+/SATB2+ ovarian mucinous cystadenocarcinoma from metastatic CRC without the patient history and clinical information.

利用 SATB2 和 CDX2 免疫组化鉴定和诊断结直肠癌
据报道,SATB2 对下消化道肿瘤具有高度特异性。SATB2 的回肠-结肠转换效应涉及与 CDX2 和 HNF4A 合作激活结肠基因,在此基础上,我们推测 SATB2 和 CDX2 可能决定结直肠癌(CRC)的特征。本研究根据 SATB2 和 CDX2 的表达情况分析了 269 例 CRC 的临床病理和免疫组化特征。具有 SATB2-和/或 CDX2-表型的 CRC 与组织分化不良相关(P<0.05)。
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来源期刊
Applied Immunohistochemistry & Molecular Morphology
Applied Immunohistochemistry & Molecular Morphology ANATOMY & MORPHOLOGY-MEDICAL LABORATORY TECHNOLOGY
CiteScore
3.20
自引率
0.00%
发文量
153
期刊介绍: ​Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist. Official Journal of the International Society for Immunohistochemisty and Molecular Morphology​.
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