IRF4 Suppresses Osteogenic Differentiation of Periodontal Ligament Stem Cells by Activating IL-18 Signaling Pathway in Periodontitis.

IF 3.2 3区生物学 Q3 MATERIALS SCIENCE, BIOMATERIALS

Advanced biology Pub Date : 2024-11-01 Epub Date: 2024-07-30 DOI:10.1002/adbi.202400099

Zhenyu Hu, Yongjie Luo, Wei Lin, Xiaolian Wu

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Abstract

The present study aims to investigate the role of interferon regulatory factor 4 (IRF4) in osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and analyze the underlying signaling of these processes. In this study, IRF4 is upregulated in periodontitis periodontal ligament tissues, as compared to healthy periodontal ligament tissues. IRF4 knockdown increases cell proliferation, decreases levels of tumor necrosis factor-alpha, interleukin-6, and interleukin-8, enhances osteogenic activity, and increases the expression of RUNX family transcription factor 2, Collagen I, and Osteocalcin in PDLSCs. The opposite results are observed in IRF4 overexpressed PDLSCs. Additionally, GSEA shows that IRF4 activates the interleukin-18 (IL-18) signaling pathway. The expressions of IL-18, B-cell translocation gene 2, interleukin-1beta, and caspase-3 are decreased by IRF4 knockdown, while increased by IRF4 overexpression. IL-18 overexpression eliminates the promoting effect of IRF4 knockdown on osteogenic differentiation of PDLSCs. In conclusion, IRF4 suppresses osteogenic differentiation of PDLSCs by activating the IL-18 signaling pathway.

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IRF4通过激活牙周炎中的IL-18信号通路抑制牙周韧带干细胞的成骨分化

本研究旨在探讨干扰素调节因子4（IRF4）在牙周韧带干细胞（PDLSCs）成骨分化中的作用，并分析这些过程的潜在信号传导。在这项研究中，与健康牙周韧带组织相比，IRF4在牙周炎牙周韧带组织中上调。IRF4敲除可增加细胞增殖，降低肿瘤坏死因子-α、白细胞介素-6和白细胞介素-8的水平，增强成骨活性，并增加PDLSCs中RUNX家族转录因子2、胶原蛋白I和骨钙素的表达。在 IRF4 过表达的 PDLSCs 中则观察到相反的结果。此外，GSEA 显示 IRF4 激活了白细胞介素-18（IL-18）信号通路。IL-18、B细胞易位基因2、白细胞介素-1beta和caspase-3的表达量在IRF4敲除后减少，而在IRF4过表达后增加。IL-18的过表达消除了IRF4敲除对PDLSCs成骨分化的促进作用。总之，IRF4通过激活IL-18信号通路抑制PDLSCs的成骨分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advanced biology Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

6.60

自引率

0.00%

发文量

130