Electroacupuncture ameliorates senile osteoporosis by promoting bone remodeling and regulating autophagy.

IF 2.4 3区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Acupuncture in Medicine Pub Date : 2024-10-01 Epub Date: 2024-07-28 DOI:10.1177/09645284241265872

Jing Liu, Jun Zhou, Jinlin Wang, Xiarong Huang, Mengjian Qu, Ying Liao, Guanghua Sun, Peirui Zhong, Jinqu Tan, Zhilu Sun

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引用次数: 0

Abstract

Objectives: Osteoporosis is widely regarded as a typical aged-related disease caused by impaired bone remodeling. This research was designed to explore the protective effects of electroacupuncture (EA) on senile osteoporosis in a rat model and investigate the underlying mechanisms.

Methods: Three-month-old rats were randomly selected as the youth group, and 24-month-old rats were randomly assigned to the elderly and EA groups. Rats in the EA group received 30 min of EA at bilateral SP10, ST36, K13 and GB34 daily, 5 days a week for 8 weeks. Bone mineral density (BMD), microstructure of the bone tissue, bone turnover biomarkers and expression level of autophagy-related proteins were detected.

Results: Compared with the elderly group, EA treatment significantly increased BMD of the femur and ameliorated the microstructure. EA treatment increased trabecular bone volume ratio (= bone volume / total volume [BV/TV]) and trabecular number (Tb.N) and decreased trabecular separation (Tb.Sp) in senile osteoporosis rats. Compared with the elderly group, the serum N-terminal telopeptide of type I collagen (NTX1) level in the EA group was lower, and the serum procollagen type I N-terminal propeptide (PINP) concentration was higher. In addition, the expression of Beclin 1, microtubule-associated protein I light chain 3 (LC3B) and P62 was inhibited in the senile osteoporosis rats after EA treatment.

Conclusions: EA can effectively alleviate aging-related bone loss and improve the microstructure of bone tissue in senile osteoporosis rats, and the regulation of autophagy might be one of the important mechanisms.

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电针通过促进骨重塑和调节自噬改善老年性骨质疏松症

目的：骨质疏松症被广泛认为是一种典型的老年性疾病，是由骨重塑障碍引起的。本研究旨在探讨电针（EA）对老年性骨质疏松症大鼠模型的保护作用及其内在机制：方法：随机选取 3 个月大的大鼠为青年组，24 个月大的大鼠为老年组和 EA 组。EA 组大鼠每天在双侧 SP10、ST36、K13 和 GB34 处接受 30 分钟的 EA，每周 5 天，连续 8 周。结果显示：与老年组相比，EA治疗组大鼠的骨矿物质密度（BMD）、骨组织的微观结构、骨转换生物标志物和自噬相关蛋白的表达水平均有所提高：结果：与老年组相比，EA治疗显著增加了股骨的骨密度，改善了骨组织的微观结构。EA治疗可增加老年骨质疏松症大鼠的骨小梁体积比（=骨体积/总体积[BV/TV]）和骨小梁数量（Tb.N），降低骨小梁分离度（Tb.Sp）。与老年组相比，EA 组血清中 I 型胶原 N 端端肽（NTX1）水平较低，血清中 I 型胶原 N 端端肽（PINP）浓度较高。此外，EA治疗后，老年性骨质疏松症大鼠Beclin 1、微管相关蛋白I轻链3（LC3B）和P62的表达均受到抑制：结论：EA能有效缓解衰老相关性骨质流失，改善老年性骨质疏松症大鼠骨组织的微观结构，自噬调节可能是其重要机制之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acupuncture in Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

4.70

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Acupuncture in Medicine aims to promote the scientific understanding of acupuncture and related treatments by publishing scientific investigations of their effectiveness and modes of action as well as articles on their use in health services and clinical practice. Acupuncture in Medicine uses the Western understanding of neurophysiology and anatomy to interpret the effects of acupuncture.