Segun Olayinka Oladipo, Adeola Oluwakemi Ayoola, Keshawn Ross, Bernard Edah, Kehinde Moruf Adelakun, Moshood K. Mustapha, Carine Adous, Béatrice Gourène, Mamadou Diop, Aristide Takoukam Kamla, Wongibe Poupezo Dieudonne, Ifeanyi Christopher Nneji, Adenike Adebukola Akinsemolu, Jean-Dominique Durand, Lotanna Micah Nneji
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非洲几内亚湾海洋黄貂鱼(Fontitrygon 属)的 DNA 条形码和形态特征描述
沿海海鳐(Fontitrygon 属)包括两个来自非洲几内亚湾的特有种和濒危种(Fontitrygon margaritella 和 Fontitrygon margarita)。然而,由于外部特征相似而缺乏可靠的诊断特征,阻碍了物种鉴定,从而限制了物种保护工作。本研究旨在通过扩增线粒体细胞色素 C 氧化酶亚单位 I(mtCOI)来检测玛格丽特拉鱼和玛格丽塔鱼的形态特征并应用 DNA 条形码工具,以准确鉴定来自尼日利亚、喀麦隆、科特迪瓦、几内亚比绍和塞内加尔沿海水域的玛格丽特拉鱼和玛格丽塔鱼。形态学评估显示,玛格丽特鱼和玛格丽特鱼在颜色模式、眼影、口形和胸径数方面存在差异。我们生成了 25 个 mtCOI 条形码序列,其中包括 F. margaritella(n = 22)和 F. margarita(n = 3)。我们在美国国家生物技术信息中心(NCBI)GenBank和生命条形码数据系统(BOLD)中的查询搜索结果显示,我们新采集的玛格丽特氏菌与全球数据库中的其他玛格丽特氏菌序列匹配,而玛格丽特氏菌的mtCOI序列则是NCBI GenBank和BOLD数据库中的新序列。基于最大似然树的系统发生聚类分析将形态种归入高度支持的单系单元。我们的研究证明了分子和形态学相结合的方法在鉴定几内亚湾近海黄貂鱼方面的潜力。我们的研究有助于未来的科学研究和制定未来的保护计划。
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