Enhancement of Non-Enzymatic Antioxidant Compounds and Expression of Rosmarinic Acid Biosynthesis-Related Genes in Melissa officinalis Using Cyanobacteria

IF 1.4 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES
Narges Moradi, Seyedeh Batool Hassani, Majid Ghorbani Nohooji, Hossein Riahi, Zeinab Shariatmadari
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引用次数: 0

Abstract

Melissa officinalis L. (Lemon balm) is a medicinal plant rich in phenolic compounds, possessing numerous health benefits. To enhance the plant’s medicinal properties and antioxidant activity, this study investigated the effect of two species of Nostoc, as nitrogen-fixing cyanobacteria, on the growth and phytochemical responses of M. officinalis. Also, several metabolites content including total phenol, total flavonoid, and rosmarinic acid (RA) were assessed. Additionally, alterations in the expression of RA biosynthesis-related genes, such as Rosmarinic acid synthase (RAS), Phenylalanine ammonia lyase (PAL), and Tyrosine amino transferase (TAT), were examined quantitatively. Pot cultivation was performed in a randomized complete block design in a greenhouse, with the treated plants irrigated with cyanobacterial suspensions at 21-day intervals for 84 days. The results demonstrated that cyanobacterial-treated plants exhibited significant improvements in root and shoot length, fresh and dry weights, leaf number, and ramification compared to the control. Additionally, there was a significant increase in the levels of hydrogen peroxide (H2O2), malondialdehyde (MDA), and proline content in treated plants, indicating an induction of oxidative stress and potential activation of antioxidant pathways. Moreover, cyanobacterial treatment significantly increased the content of total phenols and total flavonoids, as well as rosmarinic acid (RA). Also, cyanobacterial-treated plants exhibited higher expression levels of PAL and RAS genes compared to the control, suggesting an enhancement in the production of RA. In conclusion, the application of cyanobacterial elicitors positively influenced the growth and phytochemical responses of M. officinalis, leading to higher RA content and increased levels of antioxidants. This research highlights the potential of cyanobacterial bioelicitors as a cost-effective and sustainable approach to improve the medicinal quality and antioxidant activity of medicinal plants.

Graphical Abstract

The effects of cyanobacteria, Nostoc spp., on total flavonoid and rosmarinic acid (RA) content, and expression levels of some RA biosynthesis-related genes in Melissa officinalis L., RAS: rosmarinic acid synthase, PAL: phenyl alanine ammonia lyase, TAT: tyrosine amino transferase

Abstract Image

Abstract Image

利用蓝藻提高香蜂草中的非酶抗氧化化合物和香豆素酸生物合成相关基因的表达量
香蜂草(Melissa officinalis L.)是一种富含酚类化合物的药用植物,对健康有诸多益处。为了提高该植物的药用价值和抗氧化活性,本研究调查了两种固氮蓝藻 Nostoc 对 M. officinalis 的生长和植物化学反应的影响。此外,还评估了多种代谢物的含量,包括总酚、总黄酮和迷迭香酸(RA)。此外,还定量检测了迷迭香酸生物合成相关基因(如迷迭香酸合成酶(RAS)、苯丙氨酸氨裂解酶(PAL)和酪氨酸氨基转移酶(TAT))的表达变化。盆栽栽培是在温室中以随机完全区组设计进行的,每隔 21 天用蓝藻悬浮液灌溉处理过的植物一次,持续 84 天。结果表明,与对照组相比,蓝藻处理过的植株在根长和芽长、鲜重和干重、叶片数量和分支方面均有显著改善。此外,处理过的植物中过氧化氢(H2O2)、丙二醛(MDA)和脯氨酸含量也有明显增加,这表明氧化应激诱导和抗氧化途径的潜在激活。此外,蓝藻处理还能显著提高总酚、总黄酮以及迷迭香酸(RA)的含量。此外,与对照相比,蓝藻处理过的植物表现出更高的 PAL 和 RAS 基因表达水平,这表明 RA 的产生得到了增强。总之,蓝藻诱导剂的应用对M. officinalis的生长和植物化学反应产生了积极影响,从而提高了RA含量和抗氧化剂水平。这项研究强调了蓝藻生物诱导剂作为一种具有成本效益和可持续的方法来提高药用植物的药用质量和抗氧化活性的潜力。
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来源期刊
CiteScore
4.00
自引率
5.90%
发文量
122
审稿时长
>12 weeks
期刊介绍: The aim of this journal is to foster the growth of scientific research among Iranian scientists and to provide a medium which brings the fruits of their research to the attention of the world’s scientific community. The journal publishes original research findings – which may be theoretical, experimental or both - reviews, techniques, and comments spanning all subjects in the field of basic sciences, including Physics, Chemistry, Mathematics, Statistics, Biology and Earth Sciences
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