Microfluidic Barcode Biochips for High-Throughput Real-Time Biomolecule and Single-Cell Screening

IF 10.1 1区 工程技术 Q1 ENGINEERING, MULTIDISCIPLINARY
Jiaoyan Qiu, Yanbo Liang, Chao Wang, Yang Yu, Yu Zhang, Hong Liu, Lin Han
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引用次数: 0

Abstract

The real-time screening of biomolecules and single cells in biochips is extremely important for disease prediction and diagnosis, cellular analysis, and life science research. Barcode biochip technology, which is integrated with microfluidics, typically comprises barcode array, sample loading, and reaction unit array chips. Here, we present a review of microfluidics barcode biochip analytical approaches for the high-throughput screening of biomolecules and single cells, including protein biomarkers, microRNA (miRNA), circulating tumor DNA (ctDNA), single-cell secreted proteins, single-cell exosomes, and cell interactions. We begin with an overview of current high-throughput detection and analysis approaches. Following this, we outline recent improvements in microfluidic devices for biomolecule and single-cell detection, highlighting the benefits and limitations of these devices. This paper focuses on the research and development of microfluidic barcode biochips, covering their self-assembly substrate materials and their specific applications with biomolecules and single cells. Looking forward, we explore the prospects and challenges of this technology, with the aim of contributing toward the use of microfluidic barcode detection biochips in medical diagnostics and therapies, and their large-scale commercialization.
用于高通量实时生物分子和单细胞筛选的微流控条形码生物芯片
在生物芯片中对生物大分子和单细胞进行实时筛选,对于疾病预测和诊断、细胞分析和生命科学研究极为重要。条形码生物芯片技术与微流控技术相结合,通常由条形码阵列、样品装载和反应单元阵列芯片组成。在此,我们将综述用于生物大分子和单细胞高通量筛选的微流控条形码生物芯片分析方法,包括蛋白质生物标记物、微核糖核酸(miRNA)、循环肿瘤 DNA(ctDNA)、单细胞分泌蛋白、单细胞外泌体和细胞相互作用。我们首先概述了当前的高通量检测和分析方法。随后,我们概述了用于生物大分子和单细胞检测的微流控设备的最新改进,并强调了这些设备的优势和局限性。本文重点介绍微流体条形码生物芯片的研究与开发,涵盖其自组装基底材料及其在生物大分子和单细胞方面的具体应用。展望未来,我们将探讨这项技术的前景和挑战,以期为微流控条形码检测生物芯片在医学诊断和治疗中的应用及其大规模商业化做出贡献。
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来源期刊
Engineering
Engineering Environmental Science-Environmental Engineering
自引率
1.60%
发文量
335
审稿时长
35 days
期刊介绍: Engineering, an international open-access journal initiated by the Chinese Academy of Engineering (CAE) in 2015, serves as a distinguished platform for disseminating cutting-edge advancements in engineering R&D, sharing major research outputs, and highlighting key achievements worldwide. The journal's objectives encompass reporting progress in engineering science, fostering discussions on hot topics, addressing areas of interest, challenges, and prospects in engineering development, while considering human and environmental well-being and ethics in engineering. It aims to inspire breakthroughs and innovations with profound economic and social significance, propelling them to advanced international standards and transforming them into a new productive force. Ultimately, this endeavor seeks to bring about positive changes globally, benefit humanity, and shape a new future.
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