Aristea Grammoustianou, Ali Saeidi, Johan Longo, Felix Risch, Adrian M. Ionescu
{"title":"Real time detection of C reactive protein in interstitial fluid using electrochemical impedance spectroscopy, towards wearable health monitoring","authors":"Aristea Grammoustianou, Ali Saeidi, Johan Longo, Felix Risch, Adrian M. Ionescu","doi":"arxiv-2407.16734","DOIUrl":null,"url":null,"abstract":"Traditional detection methods of C-reactive protein (CRP) inflammation\nbiomarker, in blood are expensive, time-consuming and labor-intensive. Such\nexisting point-of-care CRP detection devices remain invasive, since they need\nblood sampling (finger-pricking or venous puncture). Here, we propose an\nelectrochemical impedance spectroscopy (EIS)-based sensor for the real-time,\nfast, specific, sensitive, and label-free detection of C-reactive protein in\nthe interstitial fluid (ISF) that can be accessed with minimally invasive\nmicroneedle arrays. The sensor has the potential to be integrated in a wearable\ndevice similar with the continuous glucose monitoring, that will detect CRP in\ninterstitial fluid in a non-invasive, inexpensive and straightforward manner.\nThe affinity based assay was tested in both buffer and ISF-like solution. The\nlimit of detection achieved was 0.7 ug/mL of CRP in buffer, and 0.8 ug/mL of\nCRP in ISF-like solution and the sensor shows excellent linearity up to 10\nug/mL. It is worth noting that the proposed sensor operates in low sample\nvolume (down to 5 uL), and has a response time of 100 seconds.","PeriodicalId":501378,"journal":{"name":"arXiv - PHYS - Medical Physics","volume":"15 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"arXiv - PHYS - Medical Physics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/arxiv-2407.16734","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Traditional detection methods of C-reactive protein (CRP) inflammation
biomarker, in blood are expensive, time-consuming and labor-intensive. Such
existing point-of-care CRP detection devices remain invasive, since they need
blood sampling (finger-pricking or venous puncture). Here, we propose an
electrochemical impedance spectroscopy (EIS)-based sensor for the real-time,
fast, specific, sensitive, and label-free detection of C-reactive protein in
the interstitial fluid (ISF) that can be accessed with minimally invasive
microneedle arrays. The sensor has the potential to be integrated in a wearable
device similar with the continuous glucose monitoring, that will detect CRP in
interstitial fluid in a non-invasive, inexpensive and straightforward manner.
The affinity based assay was tested in both buffer and ISF-like solution. The
limit of detection achieved was 0.7 ug/mL of CRP in buffer, and 0.8 ug/mL of
CRP in ISF-like solution and the sensor shows excellent linearity up to 10
ug/mL. It is worth noting that the proposed sensor operates in low sample
volume (down to 5 uL), and has a response time of 100 seconds.