Investigation of Expression Levels of Transcription Factor Genes in Native Sheep Breeds of Türkiye

IF 0.6 4区生物学 Q4 GENETICS & HEREDITY

Russian Journal of Genetics Pub Date : 2024-07-27 DOI:10.1134/s1022795424700364

K. Arslan, F. Daldaban, D. Bayram, M. H. Sohel, B. Akyüz, M. U. Çinar

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Abstract

This study was aimed to investigate the expression levels of transcription factor (TF) genes (MYF6, MYOD1, MYF5, MYOG) and proteins associated with muscle growth in the longissimus dorsi (LD) and gluteal (GL) muscles in sheep. For this aim, two fat-tailed sheep breeds (Akkaraman (n = 10), Awassi (n = 10)) and two thin-tailed sheep breeds (Kivircik (n = 10) and Karayaka (n = 10)) from Türkiye’s native sheep breeds were examined. The expression level was analyzed for MYF6, MYOD1, MYF5, and MYOG genes and proteins that RNAs and proteins were isolated from fresh tissues. As a result of the statistical analysis, in the LD tissue, respectively, MYOG and MYF5 genes in the Karayaka sheep breed; MYOD1 gene in Akkaraman sheep breed; MYF5 gene in Awassi sheep breed were found to be significant (P < 0.05). In GL tissue, respectively, MYOG and MYF6 genes in Akkaraman sheep breed; MYOD1 gene in Karayaka sheep breed; MYF6 gene in Akkaraman and Awassi sheep breed were significant (P < 0.05). The MYOG (fold change 6.87) and MYOD1 (fold change 15.41) genes were upregulated in the GL muscle of the fat-tailed Akkaraman sheep breed. In addition, in the thin-tailed Karayaka sheep breed, down-regulation of MYOD1 (fold change –0.22) gene in LD muscle and up-regulation of MYOD1 (fold change 6.67) gene in GL muscle was found. As a result, it can be considered that MYOG and MYOD1 genes as potential candidate genes in molecular selection studies for Akkaraman sheep breed in terms of muscle development.

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调查土耳其本土绵羊品种中转录因子基因的表达水平

摘要本研究旨在探讨转录因子（TF）基因（MYF6、MYOD1、MYF5、MYOG）以及与绵羊背长肌（LD）和臀长肌（GL）肌肉生长相关的蛋白质的表达水平。为此，研究人员对土耳其本土的两个肥尾绵羊品种（Akkaraman（n = 10）、Awassi（n = 10））和两个瘦尾绵羊品种（Kivircik（n = 10）和Karayaka（n = 10））进行了研究。从新鲜组织中分离出的 RNA 和蛋白质分析了 MYF6、MYOD1、MYF5 和 MYOG 基因和蛋白质的表达水平。统计分析结果表明，在 LD 组织中，卡拉亚卡绵羊品种的 MYOG 和 MYF5 基因、阿卡拉曼绵羊品种的 MYOD1 基因和阿瓦西绵羊品种的 MYF5 基因分别具有显著性差异（P < 0.05）。在 GL 组织中，阿卡拉曼绵羊品种的 MYOG 和 MYF6 基因、卡拉亚卡绵羊品种的 MYOD1 基因、阿卡拉曼绵羊品种和阿瓦西绵羊品种的 MYF6 基因分别具有显著性（P < 0.05）。肥尾阿卡拉曼羊种 GL 肌肉中的 MYOG（折叠变化 6.87）和 MYOD1（折叠变化 15.41）基因上调。此外，在瘦尾卡拉亚卡绵羊品种中，发现 LD 肌肉中的 MYOD1（折叠变化-0.22）基因下调，而 GL 肌肉中的 MYOD1（折叠变化 6.67）基因上调。因此，可以认为 MYOG 和 MYOD1 基因是阿卡拉曼绵羊品种在肌肉发育方面进行分子选择研究的潜在候选基因。

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来源期刊

Russian Journal of Genetics 生物-遗传学

CiteScore

1.00

自引率

33.30%

发文量

126

审稿时长

1 months

期刊介绍： Russian Journal of Genetics is a journal intended to make significant contribution to the development of genetics. The journal publishes reviews and experimental papers in the areas of theoretical and applied genetics. It presents fundamental research on genetic processes at molecular, cell, organism, and population levels, including problems of the conservation and rational management of genetic resources and the functional genomics, evolutionary genomics and medical genetics.