M. Parmar, T. P. Jaiswal, S. Bhattacharjee, S. S. Singh, A. K. Mishra
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引用次数: 0
Abstract
In modern times, cyanobacterial identification, characterization, and classification have adopted a pragmatic polyphasic approach assigning equal weight to the ecological, morphological, physiological, and molecular parameters. The paucity of data on cyanobacterial diversity across unexplored regions resulted in poor taxonomic characterization of cyanobacteria. Hence, the poorly explored North-Eastern lower Himalayan region of India was targeted and a true-branched soil inhabiting cyanobacterial strain KFS2 was isolated. Along with habitat analysis, morphological and 16S-rRNA gene-based molecular characterization of the isolated strain have been performed. The complex cellular morphology, uni-seriate to multi-seriate main filaments and branches, along with the 16S rRNA gene sequence-based phylogeny indicated affiliation of this strain with the genus Westiellopsis. Furthermore, the strain KFS2 showed unique morphological features such as the multi-seriate base of lateral branches, the presence of both intercalary and terminal heterocytes in the main filaments along with the intercalary position of heterocytes in the lateral branches, and was found to form a separate node with the Westiellopsis clade having strong bootstrap support in the 16S-rRNA gene-based phylogeny. Moreover, comparative analysis of folded secondary structures of D1-D1' and box-B in the 16S-23S ITS region of the strain also exhibited striking differences with other members of the genus. Considering all the unique morphological and molecular characteristics of the strain KFS2, it was suggested to represent a new species of the genus Westiellopsis, with the name Westiellopsis tiwarii being proposed in accordance with the International Code of Nomenclature of algae, fungi, and plants.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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