The anti-rheumatoid arthritic activity of Artemisia ordosica Krasch. (traditional Chinese/Mongolian medicine) extract in collagen-induced arthritis in rats.

IF 2.8 4区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of Pharmacy and Pharmacology Pub Date : 2024-11-04 DOI:10.1093/jpp/rgae097

Xiao-Yan Han, Ya-Ru Han, Hao-Yu Xu, Ya-Wei Hu, Xiao-Yan Yan, Guan-Hua Du, Zhan-Fei She, Bin Xiao

{"title":"The anti-rheumatoid arthritic activity of Artemisia ordosica Krasch. (traditional Chinese/Mongolian medicine) extract in collagen-induced arthritis in rats.","authors":"Xiao-Yan Han, Ya-Ru Han, Hao-Yu Xu, Ya-Wei Hu, Xiao-Yan Yan, Guan-Hua Du, Zhan-Fei She, Bin Xiao","doi":"10.1093/jpp/rgae097","DOIUrl":null,"url":null,"abstract":"Objectives: Rheumatoid arthritis (RA) seriously affects the daily life of people. The whole plant of Artemisia ordosica Krasch. (AOK) has been used in folk medicine. This study aimed to investigate the in vivo anti-RA effects of AOK extract (AOKE) on collagen-induced arthritis in rats.Methods: AOKE (400, 200, or 100 mg/kg) was administered orally to animals for 30 days. Body weight, paw swelling, arthritis index, thymus, and spleen indices, and pathological changes were assessed for effects of AOKE on RA. Furthermore, the inflammatory cytokines in rat serum were detected. In addition, the expressions of STAT3, Caspase-3, Galectin-3, and S100A9 in synovial tissue were researched using immunohistochemistry.Key findings: The AOKE significantly reduced the arthritis indices, paw swelling, spleen, and thymus indices. Meanwhile, AOKE (400 mg/kg) decreased the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-17A, and increased the level of IL-10 in rat serum. Histopathological examination showed that AOKE reduced inflammatory cell infiltration and cartilage erosion. Then, AOKE decreased the expressions of STAT3, Galectin-3, S100A9, and increased the expression of Caspase-3.Conclusion: AOKE had interesting anti-RA activity in rats, which deserved further research for the development and clinical use of this medicinal resource.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pharmacy and Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/jpp/rgae097","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: Rheumatoid arthritis (RA) seriously affects the daily life of people. The whole plant of Artemisia ordosica Krasch. (AOK) has been used in folk medicine. This study aimed to investigate the in vivo anti-RA effects of AOK extract (AOKE) on collagen-induced arthritis in rats.

Methods: AOKE (400, 200, or 100 mg/kg) was administered orally to animals for 30 days. Body weight, paw swelling, arthritis index, thymus, and spleen indices, and pathological changes were assessed for effects of AOKE on RA. Furthermore, the inflammatory cytokines in rat serum were detected. In addition, the expressions of STAT3, Caspase-3, Galectin-3, and S100A9 in synovial tissue were researched using immunohistochemistry.

Key findings: The AOKE significantly reduced the arthritis indices, paw swelling, spleen, and thymus indices. Meanwhile, AOKE (400 mg/kg) decreased the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-17A, and increased the level of IL-10 in rat serum. Histopathological examination showed that AOKE reduced inflammatory cell infiltration and cartilage erosion. Then, AOKE decreased the expressions of STAT3, Galectin-3, S100A9, and increased the expression of Caspase-3.

Conclusion: AOKE had interesting anti-RA activity in rats, which deserved further research for the development and clinical use of this medicinal resource.

查看原文本刊更多论文

艾蒿提取物对胶原蛋白诱导的大鼠类风湿关节炎的抗风湿活性(中药/蒙药）提取物对胶原蛋白诱导的大鼠关节炎的抗类风湿关节炎活性。

目的：类风湿性关节炎（RA）严重影响着人们的日常生活。艾蒿（Artemisia ordosica Krasch.(AOK）全草已被用于民间医药。本研究旨在探讨青蒿提取物（AOKE）对胶原蛋白诱导的大鼠关节炎的体内抗RA作用：方法：给动物口服 AOKE（400、200 或 100 mg/kg）30 天。评估体重、爪肿胀、关节炎指数、胸腺和脾脏指数以及病理变化，以确定 AOKE 对 RA 的影响。此外，还检测了大鼠血清中的炎症细胞因子。此外，还使用免疫组化方法研究了滑膜组织中 STAT3、Caspase-3、Galectin-3 和 S100A9 的表达：主要发现：奥凯能明显降低关节炎指数、爪肿胀、脾脏和胸腺指数。同时，奥克（400 毫克/千克）降低了大鼠血清中肿瘤坏死因子 (TNF)-α、白细胞介素 (IL)-1β、IL-6、IL-17A 的水平，提高了 IL-10 的水平。组织病理学检查显示，奥克减少了炎症细胞浸润和软骨侵蚀。此外，奥克还能减少 STAT3、Galectin-3 和 S100A9 的表达，并增加 Caspase-3 的表达：结论：AOKE 对大鼠具有有趣的抗 RA 活性，值得进一步研究，以开发这种药用资源并用于临床。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Pharmacy and Pharmacology 医学-药学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.