Moringa oleifera flowers: insights into their aroma chemistry, anti-inflammatory, antioxidant, and enzyme inhibitory properties.

IF 3.3 2区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Nouran M Fahmy, Shaimaa Fayez, Radwa Wahid Mohamed, Ahmed M Elissawy, Omayma A Eldahshan, Gokhan Zengin, Abdel Nasser B Singab
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引用次数: 0

Abstract

Background: Moringa oleifera is a highly nutritious plant widely used in traditional medicine.

Results: The aroma constituents present in the fresh flowers of M. oleifera versus the hydrodistilled oil and hexane extract were studied using GC-MS. Aldehydes were the major class detected in the fresh flowers (64.75%) with E-2-hexenal being the predominant component constituting > 50%. Alkane hydrocarbons, monoterpenes, and aldehydes constituted > 50% of the hydrodistilled oil, while alkane hydrocarbons exclusively constitute up to 65.48% of the hexane extract with heptacosane being the major component (46.2%). The cytotoxicity of the hexane extract was assessed on RAW 264.7 macrophages using the MTT assay which revealed no significant cytotoxicity at concentrations of 1 µg/mL and displayed IC50 value at 398.53 µg/mL as compared to celecoxib (anti-inflammatory drug) with IC50 value at 274.55 µg/ml. The hexane extract of Moringa flowers displayed good anti-inflammatory activity through suppression of NO, IL-6, and TNF-α in lipopolysaccharide-induced RAW 264.7 macrophages. The total phenolic and flavonoid content in the hexane extract was found to be 12.51 ± 0.28 mg GAE/g extract and 0.16 ± 0.01 mg RuE/g extract, respectively. It displayed moderate antioxidant activity as indicated by the in vitro DPPH, ABTS, CUPRAC, FRAP, and phosphomolybdenum (PBA) assays. No metal chelating properties were observed for the extract. The enzyme inhibitory potential of the hexane extract was evaluated on acetyl- and butyrylcholinesterases (for neuroprotective assessment), α-amylase and α-glucosidase (for antihyperglycemic assessment), and tyrosinase (for dermoprotective assessment) revealing promising results on cholinesterases, tyrosinase, and α-glucosidase.

Conclusion: Our findings suggested that M. oleifera leaves can be considered as a multidirectional ingredient for preparing functional applications.

油辣木花:对其芳香化学、抗炎、抗氧化和酶抑制特性的深入研究。
背景油杉是一种营养价值很高的植物,被广泛用于传统医学:使用气相色谱-质谱(GC-MS)对油橄榄鲜花、水蒸馏油和正己烷提取物中的芳香成分进行了研究。在鲜花中检测到的主要成分是醛类(64.75%),其中最主要的成分是 E-2-己烯醛,占 50%以上。烷烃、单萜烯和醛类在水蒸馏油中的含量大于 50%,而烷烃在正己烷提取物中的含量高达 65.48%,其中庚烷是主要成分(46.2%)。用 MTT 法评估了正己烷提取物对 RAW 264.7 巨噬细胞的细胞毒性,结果显示,浓度为 1 µg/mL 时,细胞毒性不明显,IC50 值为 398.53 µg/mL,而塞来昔布(消炎药)的 IC50 值为 274.55 µg/ml。通过抑制脂多糖诱导的 RAW 264.7 巨噬细胞中的 NO、IL-6 和 TNF-α,辣木花的己烷提取物显示出良好的抗炎活性。正己烷提取物中的总酚和类黄酮含量分别为 12.51 ± 0.28 毫克 GAE/克提取物和 0.16 ± 0.01 毫克 RuE/克提取物。体外 DPPH、ABTS、CUPRAC、FRAP 和磷钼(PBA)检测表明,它具有中等程度的抗氧化活性。提取物没有金属螯合特性。评估了己烷提取物对乙酰胆碱酯酶和丁酰胆碱酯酶(用于神经保护评估)、α-淀粉酶和α-葡萄糖苷酶(用于降血糖评估)以及酪氨酸酶(用于皮肤保护评估)的酶抑制潜能,结果显示对胆碱酯酶、酪氨酸酶和α-葡萄糖苷酶的抑制效果良好:我们的研究结果表明,油橄榄叶可作为一种多用途成分用于制备功能性应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Complementary Medicine and Therapies
BMC Complementary Medicine and Therapies INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
6.10
自引率
2.60%
发文量
300
审稿时长
19 weeks
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