Determination of nutrient concentration in liquid culture of cyanobacteria Nostoc sp. by capillary electrophoresis and inductively coupled plasma mass spectrometry

IF 2.6 4区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
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Abstract

In this work, we demonstrate the use of capillary electrophoresis and inductively coupled plasma mass spectrometry, as competitive methods primarily for ion chromatography, to determine changes in the concentration of small inorganic ions in the Nostoc sp. culture medium. Although macronutrients were analyzed by capillary electrophoresis with conductivity detection, micronutrients were analyzed by inductively coupled plasma mass spectrometry. The different light settings (light intensity and spectral composition) had a visible effect on the culture growth and depletion of calcium, magnesium, and phosphate ions, and iron and manganese elements when comparing the behavior under red or violet light with that under blue light.

Abstract Image

利用毛细管电泳和电感耦合等离子体质谱法测定蓝藻 Nostoc sp.
在这项工作中,我们展示了使用毛细管电泳和电感耦合等离子体质谱法(主要是离子色谱法的竞争性方法)来测定 Nostoc 培养基中无机小离子浓度的变化。虽然宏量营养元素是通过毛细管电泳和电导检测法进行分析的,但微量营养元素是通过电感耦合等离子体质谱法进行分析的。不同的光照设置(光照强度和光谱组成)对培养物的生长和钙、镁、磷酸根离子以及铁和锰元素的消耗有明显的影响,红光或紫光下的表现与蓝光下的表现相比较。
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来源期刊
Analytical biochemistry
Analytical biochemistry 生物-分析化学
CiteScore
5.70
自引率
0.00%
发文量
283
审稿时长
44 days
期刊介绍: The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.
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