Determination of nutrient concentration in liquid culture of cyanobacteria Nostoc sp. by capillary electrophoresis and inductively coupled plasma mass spectrometry

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Natália Melicherová , Tomáš Vaculovič , Radka Kočí , Martin Trtílek , Jana Lavická , František Foret
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引用次数: 0

Abstract

In this work, we demonstrate the use of capillary electrophoresis and inductively coupled plasma mass spectrometry, as competitive methods primarily for ion chromatography, to determine changes in the concentration of small inorganic ions in the Nostoc sp. culture medium. Although macronutrients were analyzed by capillary electrophoresis with conductivity detection, micronutrients were analyzed by inductively coupled plasma mass spectrometry. The different light settings (light intensity and spectral composition) had a visible effect on the culture growth and depletion of calcium, magnesium, and phosphate ions, and iron and manganese elements when comparing the behavior under red or violet light with that under blue light.

Abstract Image

利用毛细管电泳和电感耦合等离子体质谱法测定蓝藻 Nostoc sp.
在这项工作中,我们展示了使用毛细管电泳和电感耦合等离子体质谱法(主要是离子色谱法的竞争性方法)来测定 Nostoc 培养基中无机小离子浓度的变化。虽然宏量营养元素是通过毛细管电泳和电导检测法进行分析的,但微量营养元素是通过电感耦合等离子体质谱法进行分析的。不同的光照设置(光照强度和光谱组成)对培养物的生长和钙、镁、磷酸根离子以及铁和锰元素的消耗有明显的影响,红光或紫光下的表现与蓝光下的表现相比较。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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