High throughput spatial immune mapping reveals an innate immune scar in post-COVID-19 brains

IF 9.3 1区医学 Q1 CLINICAL NEUROLOGY

Acta Neuropathologica Pub Date : 2024-07-25 DOI:10.1007/s00401-024-02770-6

Marius Schwabenland, Dilara Hasavci, Sibylle Frase, Katharina Wolf, Nikolaus Deigendesch, Joerg M. Buescher, Kirsten D. Mertz, Benjamin Ondruschka, Hermann Altmeppen, Jakob Matschke, Markus Glatzel, Stephan Frank, Robert Thimme, Juergen Beck, Jonas A. Hosp, Thomas Blank, Bertram Bengsch, Marco Prinz

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引用次数: 0

Abstract

The underlying pathogenesis of neurological sequelae in post-COVID-19 patients remains unclear. Here, we used multidimensional spatial immune phenotyping and machine learning methods on brains from initial COVID-19 survivors to identify the biological correlate associated with previous SARS-CoV-2 challenge. Compared to healthy controls, individuals with post-COVID-19 revealed a high percentage of TMEM119⁺P2RY12⁺CD68⁺Iba1⁺HLA-DR⁺CD11c⁺SCAMP2⁺ microglia assembled in prototypical cellular nodules. In contrast to acute SARS-CoV-2 cases, the frequency of CD8⁺ parenchymal T cells was reduced, suggesting an immune shift toward innate immune activation that may contribute to neurological alterations in post-COVID-19 patients.

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高通量空间免疫图谱揭示了 COVID-19 后大脑中的先天性免疫疤痕。

COVID-19 后患者神经系统后遗症的潜在发病机制仍不清楚。在这里，我们利用多维空间免疫表型和机器学习方法对最初的 COVID-19 幸存者的大脑进行了分析，以确定与之前的 SARS-CoV-2 挑战相关的生物学相关性。与健康对照组相比，COVID-19 后患者的大脑中发现了高比例的 TMEM119+P2RY12+CD68+Iba1+HLA-DR+CD11c+SCAMP2+ 小胶质细胞，这些小胶质细胞聚集在原型细胞结节中。与急性 SARS-CoV-2 病例不同的是，CD8+实质 T 细胞的频率降低了，这表明免疫向先天性免疫激活转变，可能会导致 COVID-19 后患者的神经系统改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Neuropathologica 医学-病理学

CiteScore

23.70

自引率

3.90%

发文量

118

审稿时长

4-8 weeks

期刊介绍： Acta Neuropathologica publishes top-quality papers on the pathology of neurological diseases and experimental studies on molecular and cellular mechanisms using in vitro and in vivo models, ideally validated by analysis of human tissues. The journal accepts Original Papers, Review Articles, Case Reports, and Scientific Correspondence (Letters). Manuscripts must adhere to ethical standards, including review by appropriate ethics committees for human studies and compliance with principles of laboratory animal care for animal experiments. Failure to comply may result in rejection of the manuscript, and authors are responsible for ensuring accuracy and adherence to these requirements.