Integrated morphological, physiological and transcriptomic analyses reveal the responses of Toona sinensis seedlings to low-nitrogen stress

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2024-07-22 DOI:10.1016/j.ygeno.2024.110899

Hu Zhao, Miaomiao Ge, Fengzhe Zhang, Didi Du, Zilu Zhao, Cheng Shen, Qingping Hao, Min Xiao, Xiaopu Shi, Juan Wang, Mingqin Fan

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引用次数: 0

Abstract

Nitrogen is one of the most essential elements for plant growth and development. In this study, the growth, physiology, and transcriptome of Toona sinensis (A. Juss) Roem seedlings were compared between low-nitrogen (LN) and normal-nitrogen (NN) conditions. These results indicate that LN stress adversely influences T. sinensis seedling growth. The activities of key enzymes related to nitrogen assimilation and phytohormone contents were altered by LN stress. A total of 2828 differentially expressed genes (DEGs) in roots and 1547 in leaves were identified between the LN and NN treatments. A differential enrichment analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways indicated that nitrogen and sugar metabolism, flavonoid biosynthesis, plant hormone signal transduction, and ABC transporters, were strongly affected by LN stress. In summary, this research provides information for further understanding the response of T. sinensis to LN stress.

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综合形态学、生理学和转录组分析揭示了香椿幼苗对低氮胁迫的响应。

氮是植物生长和发育最基本的元素之一。本研究比较了低氮（LN）和正常氮（NN）条件下 Toona sinensis (A. Juss) Roem 幼苗的生长、生理和转录组。这些结果表明，低氮胁迫对香椿幼苗的生长有不利影响。低氮胁迫改变了与氮同化相关的关键酶的活性和植物激素含量。在 LN 和 NN 处理之间，根部和叶片中分别发现了 2828 个和 1547 个差异表达基因（DEGs）。京都基因和基因组百科全书（KEGG）通路的差异富集分析表明，氮和糖代谢、黄酮类化合物的生物合成、植物激素信号转导和ABC转运体受LN胁迫的影响很大。总之，这项研究为进一步了解中华鳖对LN胁迫的响应提供了信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.