Production of pyrimidine nucleosides in microbial systems via metabolic engineering: Theoretical analysis research and prospects

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xiangjun Zhang , Pilian Niu , Huiyan Liu , Haitian Fang
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引用次数: 0

Abstract

Pyrimidine nucleosides, as intermediate materials of significant commercial value, find extensive applications in the pharmaceutical industry. However, the current production of pyrimidine nucleosides largely relies on chemical synthesis, creating environmental problems that do not align with sustainable development goals. Recent progress in systemic metabolic engineering and synthetic biology has enabled the synthesis of natural products like pyrimidine nucleosides through microbial fermentation, offering a more sustainable alternative. Nevertheless, the intricate and tightly regulated biosynthetic pathways involved in the microbial production of pyrimidine nucleosides pose a formidable challenge. This study focuses on metabolic engineering and synthetic biology strategies aimed at enhancing pyrimidine nucleoside production. These strategies include gene modification, transcriptional regulation, metabolic flux analysis, cofactor balance optimization, and transporter engineering. Finally, this research highlights the challenges involved in the further development of pyrimidine nucleoside-producing strains and offers potential solutions in order to provide theoretical guidance for future research endeavors in this field.

通过代谢工程在微生物系统中生产嘧啶核苷:理论分析研究与前景。
嘧啶核苷作为具有重要商业价值的中间体材料,在制药业中有着广泛的应用。然而,目前嘧啶核苷的生产主要依赖化学合成,造成了环境问题,与可持续发展目标不符。系统代谢工程和合成生物学的最新进展使得通过微生物发酵合成嘧啶核苷等天然产品成为可能,从而提供了一种更具可持续性的替代方法。然而,微生物生产嘧啶核苷所涉及的生物合成途径错综复杂、调控严格,这构成了严峻的挑战。本研究侧重于旨在提高嘧啶核苷生产的代谢工程和合成生物学策略。这些策略包括基因修饰、转录调控、代谢通量分析、辅助因子平衡优化和转运工程。最后,本研究强调了进一步开发嘧啶核苷生产菌株所面临的挑战,并提出了潜在的解决方案,以期为该领域未来的研究工作提供理论指导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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