SERKs serve as co-receptors for SYR1 to trigger systemin-mediated defense responses in tomato

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hyewon Cho, Dain Seo, Minsoo Kim, Bo Eun Nam, Soyoun Ahn, Minju Kang, Geul Bang, Choon-Tak Kwon, Youngsung Joo, Eunkyoo Oh
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Abstract

Systemin, the first peptide hormone identified in plants, was initially isolated from tomato (Solanum lycopersicum) leaves. Systemin mediates local and systemic wound-induced defense responses in plants, conferring resistance to necrotrophic fungi and herbivorous insects. Systemin is recognized by the leucine-rich-repeat receptor-like kinase (LRR-RLK) receptor SYSTEMIN RECEPTOR1 (SYR1), but how the systemin recognition signal is transduced to intracellular signaling pathways to trigger defense responses is poorly understood. Here, we demonstrate that SERK family LRR-RLKs function as co-receptors for SYR1 to mediate systemin signal transduction in tomato. By using chemical genetic approaches coupled with engineered receptors, we revealed that the association of the cytoplasmic kinase domains of SYR1 with SERKs leads to their mutual trans-phosphorylation and the activation of SYR1, which in turn induces a wide range of defense responses. Systemin stimulates the association between SYR1 and all tomato SERKs (SlSERK1, SlSERK3A, and SlSERK3B). The resulting SYR1-SlSERK heteromeric complexes trigger the phosphorylation of TOMATO PROTEIN KINASE 1B (TPK1b), a receptor-like cytoplasmic kinase that positively regulates systemin responses. Additionally, upon association with SYR1, SlSERKs are cleaved by the Pseudomonas syringae effector HopB1, further supporting the finding that SlSERKs are activated by systemin-bound SYR1. Finally, genetic analysis using Slserk mutants showed that SlSERKs are essential for systemin-mediated defense responses. Collectively, these findings demonstrate that the systemin-mediated association of SYR1 and SlSERKs activates defense responses against herbivorous insects.

Abstract Image

SERKs 是 SYR1 的共受体,可触发番茄中系统素介导的防御反应。
系统素最初是从番茄(Solanum lycopersicum)叶片中分离出来的,它是在植物中发现的第一种多肽激素。系统素能介导植物局部和系统伤口诱导的防御反应,赋予植物对坏死性真菌和食草昆虫的抵抗力。系统素被亮氨酸-富重复受体样激酶(LRR-RLK)受体 SYSTEMIN RECEPTOR1(SYR1)识别,但系统素识别信号如何转导至细胞内信号通路以触发防御反应却鲜为人知。在这里,我们证明了 SERK 家族 LRR-RLK 在番茄中作为 SYR1 的共受体介导系统蛋白信号转导的功能。通过使用化学遗传方法和工程受体,我们揭示了 SYR1 的细胞质激酶结构域与 SERKs 的结合会导致它们相互反式磷酸化并激活 SYR1,进而诱导多种防御反应。Systemin 能刺激 SYR1 与所有番茄 SERK(SlSERK1、SlSERK3A 和 SlSERK3B)的结合。由此产生的 SYR1-SlSERK 异构体复合物会引发番茄蛋白激酶 1B (TPK1b)的磷酸化,TPK1b 是一种类似于受体的细胞质激酶,能积极调节系统素的反应。此外,与 SYR1 结合后,SlSERKs 会被丁香假单胞菌效应物 HopB1 裂解,这进一步支持了 SlSERKs 被系统素结合的 SYR1 激活这一发现。最后,利用 Slserk 突变体进行的遗传分析表明,SlSERKs 对于系统素介导的防御反应至关重要。总之,这些研究结果表明,由系统蛋白介导的 SYR1 和 SlSERKs 的结合激活了针对食草昆虫的防御反应。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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