Morphological and genetic identification of the gill monogenean parasite (Diclidophora merlangi) that infects Twobar Seabream Fish (Acanthopagrus bifasciatus) in the Arabian Gulf, Saudi Arabia

IF 1.1 4区 生物学 Q4 PARASITOLOGY
G. Alojayri, S. Al-Quraishy, E. Al-Shaebi, O. B. Mohammed, R. Abdel-Gaber
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Abstract

Summary Ectoparasites, particularly monogeneans, negatively affect fish health and growth. This study identified monogenean parasites in the twobar seabream, Acanthopagrus bifasciatus (Sparidae), inhabited the Arabian Gulf (Saudi Arabia). Following that, forty A. bifasciatus fish samples were visually examined for monogeneans. Parasite species were collected from the gills and then analyzed morphometrically, morphologically, and molecularly using the partial regions of the large subunit of ribosomal RNA (28S rRNA) and mitochondrial cytochrome C oxidase subunit I (COI) genes. Fish species were also identified using a DNA barcoding approach based on the COI gene. The monogenean species of Diclidophora merlangi (Diclidophoridae) were found in 45% of the fish species studied. The generic features of the Diclidophora genus distinguish this species. This species discriminated itself from congeners by having a muscular bulb with 17 grooved and recurved hooks, 218±10 (184–267) post-ovarian testes, and four pairs of pedunculated clamps of relative sizes. Partial 28S rRNA sequencing from monogeneans revealed that they grouped with members of the genus Diclidophora, forming a monophyletic group that supported the morphological descriptions. Molecular identification revealed that D. merlangi has a unique barcode made up of a COI sequence. The host identity was established as A. bifasciatus based on the COI gene sequences. Furthermore, a molecular phylogenetic study was performed to determine the phylogenetic affinity of parasite species and fish hosts. This study on Diclidophora species is considered the first record of this genus in the examined area.
从形态学和遗传学角度鉴定沙特阿拉伯阿拉伯湾中感染双髻鲷鱼(Acanthopagrus bifasciatus)的鳃单基因寄生虫(Diclidophora merlangi)。
摘要 体外寄生虫,尤其是单基因寄生虫,会对鱼类的健康和生长产生负面影响。本研究在栖息于阿拉伯湾(沙特阿拉伯)的双髻鲷(Acanthopagrus bifasciatus,Sparidae)中发现了单基因寄生虫。随后,目测了 40 个双髻鲷鱼样本,以确定是否存在单基因寄生虫。从鱼鳃中收集寄生虫种类,然后利用核糖体 RNA 大亚基(28S rRNA)和线粒体细胞色素 C 氧化酶亚基 I(COI)基因的部分区域进行形态、形态学和分子分析。还利用基于 COI 基因的 DNA 条形码方法鉴定了鱼类物种。在所研究的鱼类物种中,有 45% 发现了 Diclidophora merlangi(Diclidophoridae)的单属种。Diclidophora 属的一般特征使该物种与众不同。该鱼种与同属鱼种的区别在于其肌肉球体上有 17 个沟状和下弯的钩,218±10(184-267)个卵巢后睾丸,以及 4 对大小相对的有柄夹。部分 28S rRNA 测序结果显示,这些单系动物与双系动物组成了一个单系群,支持形态学描述。分子鉴定显示,D. merlangi 有一个由 COI 序列组成的独特条形码。根据 COI 基因序列,确定其宿主为 A. bifasciatus。此外,还进行了分子系统发育研究,以确定寄生虫物种与鱼类宿主的系统发育亲缘关系。这项关于双栉水母属的研究被认为是该地区对该属的首次记录。
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来源期刊
Helminthologia
Helminthologia 生物-动物学
CiteScore
1.80
自引率
0.00%
发文量
21
审稿时长
>12 weeks
期刊介绍: Helminthologia (HELMIN), published continuously since 1959, is the only journal in Europe that encompasses the individual and collaborative efforts of scientists working on a different topics of human, veterinary and plant helminthology. The journal responsibility is to enrich the theoretical and practical knowledge in very specific areas and thus contribute to the advancements in human and veterinary medicine and agronomy. Taking the advantage of comprehensive and multidisciplinary approaches journal still maintains its original spirit and is principal source of fresh scientific information regarding helminths, endoparasites and plant parasites. Addressing the most up-to date topics journal gained rightful and exceptional place next to the other high-quality scientific journals publishing in its field.
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