The inhibition roles of RAB23 gene in granulosa cell proliferation and progesterone synthesis of hen ovarian prehierarchical follicles.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

British Poultry Science Pub Date : 2024-07-22 DOI:10.1080/00071668.2024.2377748

D Zhang, H Wu, Y Wang, Z Xu, X Sun, S Liswaniso, N Qin, R Xu

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引用次数: 0

Abstract

1. The proliferation of granulosa cells is vital for the development and recruitment of hen ovarian prehierarchical follicles (PF). The RAB23 protein is a member of the Rab family, belonging to the GTPase family. This study studied the regulatory roles of the RAB23 gene in PF.2. The expression of RAB23 was significantly increased in granulosa cells (GC) during PF growth and was highest in GC at 6-8 mm diameter (p < 0.05). The RAB23 protein was mainly expressed in the GC, oocytes (OC) as well as somatic cells (SC) of the PF.3. The mRNA expression of FSHR, CCND1,CYP11A1, StAR and HSD3B1 was significantly increased in the siRNA RAB23 group (p < 0.05). Additionally, protein expression of FSHR, CCND1, CYP11A1, HSD3B1 was significantly increased (p < 0.05) after GC were transfected with RAB23-specific siRNA. Protein expression of StAR in the siRNA RAB23 group was numerically higher than that in the positive control (PC) and negative control (NC) groups. The GC proliferation rate and progesterone synthesis of the prehierarchical follicles in hen ovaries were markedly increased in vitro (p < 0.05).4.This study revealed that RAB23 might play an inhibitory role in GC proliferation and progesterone synthesis during the prehierarchical follicles development in vitro.

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RAB23基因对母鸡卵巢前层卵泡颗粒细胞增殖和孕酮合成的抑制作用

1.颗粒细胞的增殖对母鸡卵巢前层卵泡（PF）的发育和募集至关重要。RAB23 蛋白是 Rab 家族的成员，属于 GTPase 家族。本研究研究了 RAB23 基因在 PF 中的调控作用。在 PF 生长过程中，RAB23 在颗粒细胞（GC）中的表达显著增加，在直径为 6-8 mm 的 GC 中表达最高（p FSHR、CCND1、CYP11A1、StAR 和 HSD3B1 在 siRNA RAB23 组中显著增加（p p RAB23-specific siRNA.siRNA RAB23 组中 StAR 蛋白表达量明显高于阳性对照组（PC）和阴性对照组（NC）。母鸡卵巢前层卵泡的体外 GC 增殖率和孕酮合成明显增加（p RAB23 可能在体外前层卵泡发育过程中对 GC 增殖和孕酮合成起抑制作用）。

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来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .