Fast screening of α-glucosidase inhibitors from Ginkgo biloba leaf by using α-glucosidase immobilized on magnetic metal-organic framework

IF 2.8 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Yue Li, Hongmei Liu, Sikai Wang, Wei Fang, Xinxin Jiang, Guoqi Zhang, Yan Zhao
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Abstract

In this study, a ligand fishing method for the screening of α-glucosidase inhibitors from Ginkgo biloba leaf was established for the first time using α-glucosidase immobilized on the magnetic metal-organic framework. The immobilized α-glucosidase exhibited enhanced resistance to temperature and pH, as well as good thermal stability and reusability. Two ligands, namely quercitrin and quercetin, were screened from Ginkgo biloba leaf and identified by ultra-high performance liquid chromatography-tandem mass spectrometry. The half-maximal inhibitory concentration values for quercitrin and quercetin were determined to be 105.69 ± 0.39 and 83.49 ± 0.79 µM, respectively. Molecular docking further confirmed the strong inhibitory effect of these two ligands. The proposed approach in this study demonstrates exceptional efficiency in the screening of α-glucosidase inhibitors from complex natural medicinal plants, thus exhibiting significant potential for the discovery of antidiabetic compounds.

利用固定在磁性金属有机框架上的α-葡萄糖苷酶快速筛选银杏叶中的α-葡萄糖苷酶抑制剂
本研究利用固定在磁性金属有机框架上的α-葡萄糖苷酶,首次建立了从银杏叶中筛选α-葡萄糖苷酶抑制剂的配体捕捞法。固定化的 α-葡萄糖苷酶具有更强的耐温性和耐 pH 值性,以及良好的热稳定性和可重复使用性。研究人员从银杏叶中筛选出两种配体,即槲皮素和槲皮素,并通过超高效液相色谱-串联质谱进行了鉴定。经测定,槲皮素和槲皮素的半最大抑制浓度值分别为 105.69 ± 0.39 µM和 83.49 ± 0.79 µM。分子对接进一步证实了这两种配体的强烈抑制作用。本研究提出的方法在从复杂的天然药用植物中筛选α-葡萄糖苷酶抑制剂方面表现出了卓越的效率,因此在发现抗糖尿病化合物方面具有巨大的潜力。
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来源期刊
Journal of separation science
Journal of separation science 化学-分析化学
CiteScore
6.30
自引率
16.10%
发文量
408
审稿时长
1.8 months
期刊介绍: The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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