Fluorescence-Free Tracking of Polystyrene Microplastics in Mosquito Larvae Using Dark-Field Hyperspectral Microscopy

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-15 DOI:10.1134/S1061933X24600167

M. A. Kryuchkova, I. R. Ishmukhametov, Y. A. Frank, A. V. Simakova, V. V. Yartsev, D. A. Nadueva, A. A. Varenitsina, Y. V. Andreeva, R. F. Fakhrullin

引用次数: 0

Abstract

Using dark-field hyperspectral microscopy, we analyzed the distribution of fluorescently-labelled polystyrene microspheres with a size of 2 μm in mosquito larvae and observed the quenching of fluorescence and changes in spectral properties due to histological processing. We propose enhanced dark-field microscopy coupled with hyperspectral imaging as a versatile method for analysing polymer colloids (microplastics) biodistribution in complex tissues.

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利用暗视野超光谱显微镜无荧光跟踪蚊子幼虫体内的聚苯乙烯微塑料

摘要利用暗视野高光谱显微镜，我们分析了尺寸为 2 μm 的荧光标记聚苯乙烯微球在蚊子幼虫体内的分布，并观察了荧光淬灭和组织学处理引起的光谱特性变化。我们建议将增强型暗视野显微镜与高光谱成像相结合，作为分析聚合物胶体（微塑料）在复杂组织中生物分布的多功能方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.