Cryopreservation alters buffalo sperm kinematics and mitochondrial parameters, acrosome and intra-cellular calcium status.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2024-07-01 DOI:10.54680/fr24410110612

D. Kumar, J. S. Mehta, A. Jerome, P. Kumar, D. Kumar, S. Bhardwaj, C. Patil, R. Bala, N. Verma, EmptyYN Y Satish, M. Vimani, R. K. Sharma, P. Singh

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引用次数: 0

Abstract

BACKGROUND Little is known about the effects of different seasons on the cryopreservation success of buffalo sperm in terms of kinematics and sperm functional parameters. OBJECTIVE To study the effect of three seasons (winter, comfort and summer) and cryopreservation on sperm kinematics and functional properties in buffalo bulls. MATERIALS AND METHODS Semen ejaculates (n = 90) collected during three seasons i.e. winter (n = 30), comfort (n = 30), summer (n = 30) were evaluated for sperm kinematics and functional properties. RESULTS Sperm kinematics with respect to total (TM), progressive (PM) and rapid motility (RM) was higher (P < 0.05) in fresh sperm compared to sperm that had been frozen-thawed. Similarly, all kinematic parameters [viz. average path velocity (VAP), straight linear velocity (VSL), curvilinear velocity (VCL), beats cross frequency (BCF), lateral head displacement (ALH), linearity (LIN) and straightness (STR)] were higher (P < 0.01) at the fresh stage. With respect to season, frozen-thawed semen TM (57.67 ± 115 %), PM (50.2 ± 1.15 %) and RM (51.6 ± 1.19 %) were higher (P < 0.01) when using sperm collected during winter. The stage of cryopreservation (i.e., equilibration and freeze-thawing) also showed significant effects (P < 0.01) on mitochondrial superoxide positive status (MSPS), mitochondrial membrane potential (MMP), acrosome status and intra-cellular calcium status. CONCLUSION The season of sperm collection and cryopreservation have significant effects on buffalo bull sperm kinematics and functional properties. Doi.org/10.54680/fr24410110612.

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冷冻保存会改变水牛精子的运动学和线粒体参数、顶体和细胞内钙状态。

目的研究三个季节（冬季、舒适季节和夏季）和冷冻保存对水牛精子运动学和功能特性的影响。结果新鲜精子的总运动（TM）、渐进运动（PM）和快速运动（RM）与冷冻解冻精子相比更高（P < 0.05）。同样，所有运动学参数[即平均路径速度（VAP）、直线速度（VSL）、曲线速度（VCL）、节拍交叉频率（BCF）、侧头位移（ALH）、线性度（LIN）和直线度（STR）]在新鲜阶段都更高（P < 0.01）。就季节而言，使用冬季采集的精子时，冷冻解冻精液的TM（57.67 ± 115 %）、PM（50.2 ± 1.15 %）和RM（51.6 ± 1.19 %）较高（P < 0.01）。冷冻保存阶段（即平衡和冻融）对线粒体超氧化物阳性状态（MSPS）、线粒体膜电位（MMP）、顶体状态和细胞内钙状态也有显著影响（P < 0.01）。Doi.org/10.54680/fr24410110612.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.