Methods of Analysis of Phytoestrogenic Compounds: An Up-to-Date of the Present State

IF 2.5 4区工程技术 Q3 CHEMISTRY, ANALYTICAL

Separations Pub Date : 2024-07-01 DOI:10.3390/separations11070205

Ines Adam-Dima, Andreea Alexandra Olteanu, O. T. Olaru, Daniela Elena Popa, C. Purdel

引用次数: 0

Abstract

Phytoestrogens, natural compounds with structural similarity to 17-β-estradiol, are known to have potential health benefits, including in hormone-dependent malignancies. The therapeutic interest and some safety concerns observed triggered the need to develop accurate methods to assess their level in different matrices. This narrative review examines the existing analytical methods used to isolate, quantify, and characterize phytoestrogens and their metabolites in plants, foods, and biological samples. Different conventional and modern extraction techniques, such as ultrasonication-assisted extraction, supercritical fluid extraction, or enzyme-assisted extraction, were presented and compared. The advantages and limitations of the existing analytical methods, such as high-performance liquid chromatography using different sensitive detectors, gas chromatography often coupled with mass spectrometry, and immunoassay methods, are highlighted, along with the need for ongoing research to improve the sensitivity and selectivity of the analysis.

查看原文本刊更多论文

植物雌激素化合物的分析方法：最新现状

众所周知，植物雌激素是与 17-β-estradiol 结构相似的天然化合物，具有潜在的健康益处，包括对激素依赖性恶性肿瘤的益处。对治疗的兴趣和对安全性的担忧促使人们需要开发精确的方法来评估它们在不同基质中的含量。本综述探讨了用于植物、食品和生物样本中植物雌激素及其代谢物的分离、定量和定性的现有分析方法。综述介绍并比较了不同的传统和现代萃取技术，如超声辅助萃取、超临界流体萃取或酶辅助萃取。重点介绍了现有分析方法的优势和局限性，如使用不同灵敏度检测器的高效液相色谱法、通常与质谱联用的气相色谱法和免疫测定法，以及提高分析灵敏度和选择性的持续研究需求。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Separations Chemistry-Analytical Chemistry

CiteScore

3.00

自引率

15.40%

发文量

342

审稿时长

12 weeks

期刊介绍： Separations (formerly Chromatography, ISSN 2227-9075, CODEN: CHROBV) provides an advanced forum for separation and purification science and technology in all areas of chemical, biological and physical science. It publishes reviews, regular research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: Manuscripts regarding research proposals and research ideas will be particularly welcomed. Electronic files and software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. Manuscripts concerning summaries and surveys on research cooperation and projects (that are funded by national governments) to give information for a broad field of users. The scope of the journal includes but is not limited to: Theory and methodology (theory of separation methods, sample preparation, instrumental and column developments, new separation methodologies, etc.) Equipment and techniques, novel hyphenated analytical solutions (significantly extended by their combination with spectroscopic methods and in particular, mass spectrometry) Novel analysis approaches and applications to solve analytical challenges which utilize chromatographic separations as a key step in the overall solution Computational modelling of separations for the purpose of fundamental understanding and/or chromatographic optimization