Herbal emulgel containing Azadirachta indica (Neem) and Nigella Sativa L. (Black Cumin) oils in wound management: Preclinical investigations

Asha Thomas, Madhura Dapkekar, D. Nagore, Rohit Doke, Nilam Bankar, Namita S. Surve
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引用次数: 1

Abstract

Context: In wound management, traditional use of plants has received attention by the scientific community, as traditional medicines are a valuable source of affordable, comprehensive medical care. Azadirachta indica A. Juss (Neem) and Nigella sativa Linn (Black cumin) are well-known versatile medicinal plants with wide spectrum of biological activity. Methods: This study was undertaken to evaluate the wound healing activity of neem oil (NO) and black cumin oil (BCO) in rats. The oils were standardized using standard procedures. Gas chromatography mass spectrometry analysis and high-performance thin-layer chromatography fingerprinting of oils were carried out to identify major phytoconstituents present in the oils. The oils were suitably incorporated in carbopol-based emulgel formulation, evaluated for physicochemical parameters and stability. Results: The wound healing potential assessed by the measurement of wound contraction and histopathology studies in excision and burn wound models, demonstrated comparable efficacy with standard nano-crystalline silver gel (0.002% w/w). This may be attributed to the presence of phytoconstituents such as nimbidin and thymoquinone present in selected oils. Polyunsaturated fatty acid profile of BCO may be responsible for stimulating the release of vascular endothelial growth factor, which plays a significant role in accelerating wound healing process. Conclusion: Topical carbopol-934 base emulgel containing natural oils such as BCO and NO have been successfully prepared for wound management. However, further studies to establish the mechanistic aspects of wound healing needs to be undertaken.
含有印度楝树油(Azadirachta indica)和黑小茴香油(Nigella Sativa L. (Black Cumin) oils)的草药凝胶在伤口管理中的应用:临床前研究
背景:在伤口处理方面,传统植物的使用受到科学界的关注,因为传统药物是负担得起的综合医疗保健的宝贵来源。Azadirachta indica A. Juss(印度楝树)和 Nigella sativa Linn(黑小茴香)是著名的多用途药用植物,具有广泛的生物活性。研究方法本研究旨在评估印楝油(NO)和黑孜然油(BCO)对大鼠伤口愈合的活性。采用标准程序对这两种油进行了标准化处理。对油进行了气相色谱质谱分析和高性能薄层色谱指纹图谱分析,以确定油中的主要植物成分。将这些精油适当地加入到以 carbopol 为基础的凝胶配方中,并对其理化参数和稳定性进行了评估。结果:通过对割伤和烧伤模型进行伤口收缩测量和组织病理学研究,对伤口愈合潜力进行了评估,结果表明其疗效与标准纳米结晶银凝胶(0.002% w/w)相当。这可能归功于所选油中含有的植物成分,如 nimbidin 和 thymoquinone。BCO 中的多不饱和脂肪酸成分可能会刺激血管内皮生长因子的释放,而血管内皮生长因子在加速伤口愈合过程中发挥着重要作用。结论含有BCO和NO等天然油脂的外用carbopol-934基质凝胶已成功制备用于伤口管理。不过,还需要进一步研究伤口愈合的机理。
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