Assembly and Annotation of the Complete Genome Sequence of the Paenibacillus Bacteriophage phJNUCC32

Q4 Medicine
Yang Xu, Xuhui Liang, Chang-Gu Hyun
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引用次数: 0

Abstract

A potential biocontrol agent for American foulbrood (AFB), the Paenibacillus bacteriophage phJNUCC32, was isolated from Baengnokdam in Halla Mountain. This study aimed to investigate its genomic characteristics through whole-genome sequencing. The genome of phJNUCC32 was found to be 62,871 base pairs in length, with a G + C content of 51.98%. Phylogenetic analysis classified phJNUCC32 within the unclassified Caudoviricetes bacteriophage category. The genome prediction confirmed the absence of virulence factors and antibiotic-resistance genes, ensuring its genetic safety. A total of 63 coding DNA sequences were identified, revealing a modular arrangement. Notably, the annotation of gene function indicates that phJNUCC32 harbors the holin/lysin system, suggesting significant potential for controlling bacterial infections in AFB and agriculture.
噬菌体 phJNUCC32 完整基因组序列的组装与注释
从哈拉山的 Baengnokdam 分离出了一种潜在的美国恶臭病(AFB)生物控制剂--Paenibacillus 噬菌体 phJNUCC32。本研究旨在通过全基因组测序研究其基因组特征。研究发现,噬菌体 phJNUCC32 的基因组长度为 62,871 个碱基对,G+C 含量为 51.98%。系统发生分析将 phJNUCC32 归入未分类的 Caudoviricetes 噬菌体类别。基因组预测证实phJNUCC32不含毒力因子和抗生素抗性基因,确保了其基因安全性。共鉴定出 63 个编码 DNA 序列,显示出模块化排列。值得注意的是,基因功能注释表明 phJNUCC32 含有 holin/lysin 系统,这表明它在控制 AFB 和农业中的细菌感染方面具有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta Microbiologica Hellenica
Acta Microbiologica Hellenica Medicine-Microbiology (medical)
CiteScore
0.20
自引率
0.00%
发文量
6
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