Prevalence Studies on Brucellosis in Buffalo Population in Nearby Region of Hyderabad, Telangana

Asian Journal of Dairy and Food Research Pub Date : 2024-07-08 DOI:10.18805/ajdfr.dr-2140

A. Pradeep, Sujatha Singh, A. Kumar, P. Kalyani, N. Krishnaiah, G. Lavleen

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Abstract

Background: Brucellosis in India is highly endemic in many states and reported as the most prevalent disease in dairy cattle. Brucellosis is a highly contagious bacterial disease caused by various Brucella species mainly affecting wide range of different species of animals like cattle, sheep, goat and pigs. The present study was undertaken to ascertain the prevalence of brucellosis in suspected buffalo population showing reproductive disorders in selected dairy farms residing nearby region of Hyderabad, Telangana. Methods: Total number of 204 vaginal swab samples were collected aseptically from suspected buffaloes in nearby private dairy farms and were screened by employing various diagnostic methods. Enzyme Linked Immuno Sorbent Assay (ELISA), Lateral Flow Assay (LFA) and molecular identification through Polymerase Chain Reaction (PCR) were conducted. The sensitivity of tests was recorded considering PCR as gold standard method. Result: Screening of 204 vaginal swab samples and prevalence of brucellosis were recorded by ensuring the positive reactions and also depicting the sensitivity of various diagnostic tests namely ELISA, LFA and PCR accounting 92(45.1%), 87 (42.64%) and 113(55.4%) samples respectively. The specificity of both LFA and ELISA were recorded 100% when compared to PCR assay.

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特伦甘纳邦海德拉巴附近地区水牛布鲁氏菌病流行情况研究

背景：布鲁氏菌病在印度许多邦高度流行，据报道是奶牛最常见的疾病。布鲁氏菌病是一种高度传染性的细菌性疾病，由多种布鲁氏菌引起，主要影响牛、绵羊、山羊和猪等不同种类的动物。本研究旨在确定居住在泰兰加纳州海德拉巴附近地区的选定奶牛场中出现繁殖障碍的疑似水牛群体中布鲁氏杆菌病的流行情况。研究方法从附近私人奶牛场的疑似水牛身上无菌采集了 204 份阴道拭子样本，并采用各种诊断方法进行筛查。采用酶联免疫吸附试验（ELISA）、侧流试验（LFA）和聚合酶链式反应（PCR）进行分子鉴定。将聚合酶链式反应作为金标准方法，记录了测试的灵敏度。结果对 204 份阴道拭子样本进行了筛查，并通过确保阳性反应记录了布鲁氏菌病的流行情况，同时还描述了各种诊断检测的灵敏度，即 ELISA、LFA 和 PCR 分别占 92（45.1%）、87（42.64%）和 113（55.4%）份样本。与 PCR 检测相比，LFA 和 ELISA 的特异性均达到 100%。

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Asian Journal of Dairy and Food Research

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