Biomedical Promise of Aspergillus Flavus-Biosynthesized Selenium Nanoparticles: A Green Synthesis Approach to Antiviral, Anticancer, Anti-Biofilm, and Antibacterial Applications

Pharmaceuticals Pub Date : 2024-07-09 DOI:10.3390/ph17070915
E. Mohammed, Ahmed E. M. Abdelaziz, Alsayed E. Mekky, Nashaat N. Mahmoud, Mohamed Sharaf, Mahmoud M. Al-Habibi, Nehal M. Khairy, A. Al-Askar, F. Youssef, Mahmoud Ali Gaber, Ebrahim Saied, Gehad AbdElgayed, S. A. Metwally, Aly A. Shoun
{"title":"Biomedical Promise of Aspergillus Flavus-Biosynthesized Selenium Nanoparticles: A Green Synthesis Approach to Antiviral, Anticancer, Anti-Biofilm, and Antibacterial Applications","authors":"E. Mohammed, Ahmed E. M. Abdelaziz, Alsayed E. Mekky, Nashaat N. Mahmoud, Mohamed Sharaf, Mahmoud M. Al-Habibi, Nehal M. Khairy, A. Al-Askar, F. Youssef, Mahmoud Ali Gaber, Ebrahim Saied, Gehad AbdElgayed, S. A. Metwally, Aly A. Shoun","doi":"10.3390/ph17070915","DOIUrl":null,"url":null,"abstract":"This study utilized Aspergillus flavus to produce selenium nanoparticles (Se-NPs) in an environmentally friendly and ecologically sustainable manner, targeting several medicinal applications. These biosynthesized Se-NPs were meticulously characterized using X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, transmission electron microscope (TEM), and UV–visible spectroscopy (UV), revealing their spherical shape and size ranging between 28 and 78 nm. We conducted further testing of Se-NPs to evaluate their potential for biological applications, including antiviral, anticancer, antibacterial, antioxidant, and antibiofilm activities. The results indicate that biosynthesized Se-NPs could be effective against various pathogens, including Salmonella typhimurium (ATCC 14028), Bacillus pumilus (ATCC 14884), Staphylococcus aureus (ATCC 6538), Clostridium sporogenes (ATCC 19404), Escherichia coli (ATCC 8739), and Bacillus subtilis (ATCC 6633). Additionally, the biosynthesized Se-NPs exhibited anticancer activity against three cell lines: pancreatic carcinoma (PANC1), cervical cancer (Hela), and colorectal adenocarcinoma (Caco-2), with IC50 values of 177, 208, and 216 μg/mL, respectively. The nanoparticles demonstrated antiviral activity against HSV-1 and HAV, achieving inhibition rates of 66.4% and 15.1%, respectively, at the maximum non-toxic concentration, while also displaying antibiofilm and antioxidant properties. In conclusion, the biosynthesized Se-NPs by A. flavus present a promising avenue for various biomedical applications with safe usage.","PeriodicalId":509865,"journal":{"name":"Pharmaceuticals","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmaceuticals","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/ph17070915","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

This study utilized Aspergillus flavus to produce selenium nanoparticles (Se-NPs) in an environmentally friendly and ecologically sustainable manner, targeting several medicinal applications. These biosynthesized Se-NPs were meticulously characterized using X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, transmission electron microscope (TEM), and UV–visible spectroscopy (UV), revealing their spherical shape and size ranging between 28 and 78 nm. We conducted further testing of Se-NPs to evaluate their potential for biological applications, including antiviral, anticancer, antibacterial, antioxidant, and antibiofilm activities. The results indicate that biosynthesized Se-NPs could be effective against various pathogens, including Salmonella typhimurium (ATCC 14028), Bacillus pumilus (ATCC 14884), Staphylococcus aureus (ATCC 6538), Clostridium sporogenes (ATCC 19404), Escherichia coli (ATCC 8739), and Bacillus subtilis (ATCC 6633). Additionally, the biosynthesized Se-NPs exhibited anticancer activity against three cell lines: pancreatic carcinoma (PANC1), cervical cancer (Hela), and colorectal adenocarcinoma (Caco-2), with IC50 values of 177, 208, and 216 μg/mL, respectively. The nanoparticles demonstrated antiviral activity against HSV-1 and HAV, achieving inhibition rates of 66.4% and 15.1%, respectively, at the maximum non-toxic concentration, while also displaying antibiofilm and antioxidant properties. In conclusion, the biosynthesized Se-NPs by A. flavus present a promising avenue for various biomedical applications with safe usage.
黄曲霉菌生物合成硒纳米粒子的生物医学前景:抗病毒、抗癌、抗生物膜和抗菌应用的绿色合成方法
本研究利用黄曲霉以环境友好和生态可持续的方式生产硒纳米粒子(Se-NPs),目标是多种药物应用。我们使用 X 射线衍射(XRD)、傅立叶变换红外光谱(FT-IR)、透射电子显微镜(TEM)和紫外可见光谱(UV)对这些生物合成的 Se-NPs 进行了细致的表征,发现它们呈球形,大小在 28 纳米到 78 纳米之间。我们对 Se-NPs 进行了进一步测试,以评估它们在生物应用方面的潜力,包括抗病毒、抗癌、抗菌、抗氧化和抗生物膜活性。结果表明,生物合成的Se-NPs可有效对抗各种病原体,包括鼠伤寒沙门氏菌(ATCC 14028)、枯草杆菌(ATCC 14884)、金黄色葡萄球菌(ATCC 6538)、产气荚膜梭菌(ATCC 19404)、大肠杆菌(ATCC 8739)和枯草杆菌(ATCC 6633)。此外,生物合成的 Se-NPs 对胰腺癌(PANC1)、宫颈癌(Hela)和结直肠腺癌(Caco-2)这三种细胞系具有抗癌活性,IC50 值分别为 177、208 和 216 μg/mL。纳米颗粒对 HSV-1 和 HAV 具有抗病毒活性,在最大无毒浓度下的抑制率分别为 66.4% 和 15.1%,同时还具有抗生物膜和抗氧化特性。总之,黄曲霉菌生物合成的Se-NPs为各种生物医学应用提供了一个安全使用的前景广阔的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信