Quantitative and ultrasensitive detection of four inflammatory biomarkers by gold nanoparticle‐based paper sensor

IF 2.6 4区材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY

ChemNanoMat Pub Date : 2024-07-15 DOI:10.1002/cnma.202400118

liya ye, aihong wu, Liqiang Liu, Hua Kuang, Chuanlai Xu, Xinxin Xu

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Abstract

Four inflammatory biomarkers (CRP, IL‐6, PCT and SAA) have different manifestations and roles in different infectious diseases and stages of infection, and the diagnosis and identification of infectious diseases can be realized through quantitative analysis of these four biomarkers simultaneously. In this study, we developed a colloidal gold nanoparticle (AuNP)‐based multiplex LFA strip using eight highly‐sensitive monoclonal antibodies that simultaneously detected CRP, IL‐6, PCT and SAA in serum within 15 min. And quantitative analysis was achieved with a portable strip reader, the LODs were 2.5 μg/mL, 25.3 pg/mL 0.87 ng/mL and 8.8 μg/mL for CRP, IL‐6, PCT and SAA, while detection ranges were 2.5–200 μg/mL, 25.3–8000 pg/mL 0.87–100 ng/mL and 8.8–200 μg/mL. Moreover, the quantitative colloidal gold assay correlated well with the results of a chemiluminescence immunoassay when testing clinical serum samples. Our developed method was reliable and accurate according to the recovery test results. Therefore, the strip can be used as an alternative method for the simultaneous monitoring of CRP, IL‐6, PCT and SAA in serum samples.

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基于金纳米粒子的纸质传感器对四种炎症生物标记物进行定量和超灵敏检测

四种炎症生物标志物（CRP、IL-6、PCT 和 SAA）在不同的感染性疾病和感染阶段有不同的表现和作用，通过同时定量分析这四种生物标志物可以实现对感染性疾病的诊断和鉴别。本研究开发了一种基于胶体金纳米颗粒（AuNP）的多重 LFA 检测试纸条，使用八种高灵敏度的单克隆抗体，可在 15 分钟内同时检测血清中的 CRP、IL-6、PCT 和 SAA。CRP、IL-6、PCT 和 SAA 的检测限分别为 2.5 μg/mL、25.3 pg/mL 0.87 ng/mL 和 8.8 μg/mL，检测范围分别为 2.5-200 μg/mL、25.3-8000 pg/mL 0.87-100 ng/mL 和 8.8-200 μg/mL。此外，在检测临床血清样本时，胶体金定量检测法与化学发光免疫分析法的结果具有良好的相关性。根据回收率测试结果，我们开发的方法是可靠和准确的。因此，该试剂条可作为同时监测血清样本中 CRP、IL-6、PCT 和 SAA 的替代方法。

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来源期刊

ChemNanoMat Energy-Energy Engineering and Power Technology

CiteScore

6.10

自引率

2.60%

发文量

236

期刊介绍： ChemNanoMat is a new journal published in close cooperation with the teams of Angewandte Chemie and Advanced Materials, and is the new sister journal to Chemistry—An Asian Journal.

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