Development and Validation of a High-Performance Liquid Chromatography Diode Array Detector Method to Measure Seven Neonicotinoids in Wheat

Foods Pub Date : 2024-07-16 DOI:10.3390/foods13142235
S. Seccia, Stefania Albrizio, Elena Morelli, I. Dini
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Abstract

Neonicotinoids (NEOs), used as insecticides against aphids, whiteflies, lepidopterans, and beetles, have numerous detrimental impacts on human health, including chronic illnesses, cancer, infertility, and birth anomalies. Monitoring the residues in food products is necessary to guarantee public health and ecological balance. The present work validated a new method to measure seven neonicotinoid insecticides (acetamiprid ACT, clothianidin CLT, dinotefuran DNT, imidacloprid IMD, nitenpyram NTP, thiacloprid TCP, and thiamethoxan THT) in wheat. The analytical procedure was based on simple and fast wheat sample cleanup using solid-phase extraction (SPE) to remove interferents and enrich the NEOs, alongside the NEOs’ separation and quantification by reverse-phase chromatography coupled with a diode array detector (DAD). The validation process was validated using the accuracy profile strategy, a straightforward decision tool based on the measure of the total error (bias plus standard deviation) of the method. Our results proved that, in the future, at least 95% of the results obtained with the proposed method would fall within the ±15% acceptance limits. The test’s cost-effectiveness, rapidity, and simplicity suggest its use for determining the levels of acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid, and thiamethoxam in routine analyses of wheat.
开发并验证测量小麦中七种新烟碱的高效液相色谱二极管阵列检测器方法
新烟碱类(NEOs)作为杀虫剂用于防治蚜虫、粉虱、鳞翅目昆虫和甲虫,对人类健康有诸多不利影响,包括慢性疾病、癌症、不孕不育和先天畸形。为了保证公众健康和生态平衡,有必要对食品中的残留进行监测。本研究验证了一种测定小麦中七种新烟碱类杀虫剂(啶虫脒 ACT、噻虫嗪 CLT、乐果 DNT、吡虫啉 IMD、硝虫啉 NTP、噻虫啉 TCP 和噻虫嗪 THT)的新方法。分析过程基于简单快速的小麦样品净化,使用固相萃取(SPE)去除干扰物并富集近地天敌,同时使用反相色谱法结合二极管阵列检测器(DAD)对近地天敌进行分离和定量。验证过程采用了准确度曲线策略,这是一种基于测量方法总误差(偏差加标准偏差)的直接决策工具。我们的结果证明,今后至少有 95% 的结果将在 ±15% 的接受范围内。该检测方法具有成本效益高、快速、简便等特点,建议用于测定小麦常规分析中啶虫脒、噻虫嗪、敌稗、吡虫啉、硝虫啉、噻虫啉和噻虫嗪的含量。
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