Use of two different methods for glucose determination in sheep under normoglycemic, hypoglycemic, and hyperglycemic conditions: an evaluation of practical diagnostic methods in ovines

IF 1.3 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Production Science Pub Date : 2024-07-16 DOI:10.1071/an24165

M. G. Chenard, Jade de Menezes Paes Bastos, Matheus Dias Valle, Isabelle Magalhães da Cunha, N. Alencar, Daniel Augusto Barroso Lessa, Guilherme Nunes de Souza, Nathalie Costa da Cunha, M. A. Helayel

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引用次数: 0

Abstract

Context Animals can present abnormal blood glucose concentrations because of various diseases or pathological conditions, stress, or hunger. Early diagnosis prevents complications, economic losses, and death. The use of a portable glucometer (PGM) has been shown to be a good, simple, and practical alternative method with good precision and accuracy for assessing blood glucose in humans and companion animals. Aims The objective of this work was to evaluate the accuracy and reliability of a portable glucometer (PGM) for assessing glycemia in normoglycemic, hypoglycemic, and hyperglycemic sheep. Methods Blood glucose was evaluated in 60 normoglycemic, 15 hypoglycemic, and 15 hyperglycemic sheep. Blood samples were collected and analysed within 2 h by using PGM and the enzymatic method (EM). Each test was evaluated for sensitivity, specificity, and the area under the receiver operating characteristic (ROC) curve for two cutoff points, namely, one for hypoglycemia and the other for hyperglycemia. Key results The results of the Kolmogorov–Smirnov test (P < 0.05) for all groups evaluated did not show a normal distribution for the values evaluated by PGM and EM. Despite the significant difference found between the medians of the methods and the low homogeneity according to the coefficient of variation (CV), there was a homogeneous and linear dispersion of the results. The Bland–Altman test showed that the mean difference between the two methods was close to zero, denoting good agreement, precision, and accuracy of PGM when compared to EM. Conclusions PGM presents high accuracy and precision for assessing glycemia in sheep, providing satisfactory and reliable results when compared with EM. Implications The use of PGM facilitates the veterinarian’s routine, promoting early diagnosis, field examinations, and monitoring of metabolic diseases.

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在正常血糖、低血糖和高血糖条件下使用两种不同方法测定绵羊的葡萄糖：对绵羊实用诊断方法的评估

背景动物会因各种疾病或病理状况、压力或饥饿而导致血糖浓度异常。早期诊断可预防并发症、经济损失和死亡。使用便携式血糖仪（PGM）已被证明是一种良好、简单、实用的替代方法，具有良好的精确性和准确性，可用于评估人类和伴侣动物的血糖。目的评估便携式血糖仪（PGM）评估血糖正常、血糖过低和血糖过高绵羊血糖的准确性和可靠性。方法对 60 只正常血糖绵羊、15 只低血糖绵羊和 15 只高血糖绵羊的血糖进行评估。采集血样并在 2 小时内用 PGM 和酶法（EM）进行分析。对每种检测方法的灵敏度、特异性以及两个截断点（一个为低血糖，另一个为高血糖）的接收器操作特征曲线下面积进行了评估。主要结果对所有评估组进行的 Kolmogorov-Smirnov 检验（P < 0.05）结果显示，PGM 和 EM 评估值不呈正态分布。尽管这两种方法的中位数之间存在明显差异，而且根据变异系数（CV）计算的同质性较低，但结果仍呈均匀的线性分布。Bland-Altman 检验表明，两种方法的平均差异接近零，这表明与 EM 相比，PGM 具有良好的一致性、精确性和准确性。结论 PGM 在评估绵羊血糖方面具有较高的准确性和精确度，与 EM 相比可提供令人满意的可靠结果。意义使用 PGM 方便了兽医的日常工作，促进了代谢疾病的早期诊断、现场检查和监测。

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来源期刊

Animal Production Science AGRICULTURE, MULTIDISCIPLINARY-

自引率

7.10%

发文量

139

期刊介绍： Research papers in Animal Production Science focus on improving livestock and food production, and on the social and economic issues that influence primary producers. The journal (formerly known as Australian Journal of Experimental Agriculture) is predominantly concerned with domesticated animals (beef cattle, dairy cows, sheep, pigs, goats and poultry); however, contributions on horses and wild animals may be published where relevant. Animal Production Science is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.